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Catalog Number Size Price
LS-C343986-100 100 µg (0.5 mg/ml) $355 
ACE / CD143 Antibody - IHC analysis of ACE using anti-ACE antibody. ACE was detected in frozen section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ACE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ACE / CD143 Antibody - ACE antibody IHC-paraffin: Rat Lung Tissue.
ACE / CD143 Antibody - IHC analysis of ACE using anti-ACE antibody. ACE was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ACE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ACE / CD143 Antibody - ACE antibody IHC-paraffin: Rat Lung Tissue.
ACE / CD143 Antibody - ACE antibody IHC-paraffin: Human Placenta Tissue.
ACE / CD143 Antibody - ACE antibody IHC-paraffin: Mouse Lung Tissue.
ACE / CD143 Antibody - ACE antibody Western blot. All lanes: Anti ACE at 0.5 ug/ml. WB: Recombinant Human ACE Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.
ACE / CD143 Antibody - Western blot analysis of ACE using anti-ACE antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACE at approximately 180KD. The expected band size for ACE is at 150KD.
ACE / CD143 Antibody - IHC analysis of ACE using anti-ACE antibody. ACE was detected in frozen section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ACE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ACE / CD143 Antibody - ACE antibody IHC-paraffin: Rat Lung Tissue.
ACE / CD143 Antibody - IHC analysis of ACE using anti-ACE antibody. ACE was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ACE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ACE / CD143 Antibody - ACE antibody IHC-paraffin: Rat Lung Tissue.
ACE / CD143 Antibody - ACE antibody IHC-paraffin: Human Placenta Tissue.
ACE / CD143 Antibody - ACE antibody IHC-paraffin: Mouse Lung Tissue.
ACE / CD143 Antibody - ACE antibody Western blot. All lanes: Anti ACE at 0.5 ug/ml. WB: Recombinant Human ACE Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.
ACE / CD143 Antibody - Western blot analysis of ACE using anti-ACE antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACE at approximately 180KD. The expected band size for ACE is at 150KD.
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ACE / CD143 Antibody (aa651‑864) LS‑C343986

ACE / CD143 Antibody (aa651‑864) LS‑C343986

Note: This antibody replaces LS-C387929
Antibody:
Rabbit Polyclonal to Human ACE / CD143
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$355 
LS-C343986-100
100 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ACE / CD143
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD143 antibody LS-C343986 is an unconjugated rabbit polyclonal antibody to CD143 (ACE ) from human, mouse and rat. Validated for IHC and WB.
Target
Human ACE / CD143
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E.coli-derived human ACE recombinant protein (Position: K651-Y864). Human ACE shares 73% and 76% amino acid (aa) sequences identity with mouse and rat ACE, respectively.
Epitope
aa651-864
Specificity
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .
Presentation
Contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2 HPO4, 0.05 mg sodium azide per 100 ug antibody.
Reconstitution
200 µl Distilled water
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACE / CD143
P12821 NM_000789 NP_000780.1

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Requested From: United States
Date Requested: 1/19/2020