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Catalog Number	Size	Price
LS-C343986-10	10 µg	$318
LS-C343986-100	100 µg (0.5 mg/ml)	$470

ACE / CD143 Antibody - ACE antibody IHC-paraffin: Rat Lung Tissue.

ACE / CD143 Antibody - IHC analysis of ACE using anti-ACE antibody. ACE was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ACE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

ACE / CD143 Antibody - IHC analysis of ACE using anti-ACE antibody. ACE was detected in frozen section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ACE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

ACE / CD143 Antibody - ACE antibody IHC-paraffin: Human Placenta Tissue.

ACE / CD143 Antibody - ACE antibody IHC-paraffin: Mouse Lung Tissue.

ACE / CD143 Antibody - Western blot analysis of ACE using anti-ACE antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACE at approximately 180KD. The expected band size for ACE is at 150KD.

ACE / CD143 Antibody - ACE antibody Western blot. All lanes: Anti ACE at 0.5 ug/ml. WB: Recombinant Human ACE Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.

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Polyclonal Rabbit anti‑Human ACE / CD143 Antibody (aa651‑864, IHC, WB) LS‑C343986

Name: Anti-ACE / CD143 Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C343986-10
Price: 318.0000 USD
Availability: InStock

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Note: This antibody replaces LS-C387929

Antibody:

ACE / CD143 Rabbit anti-Human Polyclonal (aa651-864) Antibody

Application:

IHC, IHC-P, IHC-Fr, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C343986-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ACE / CD143 Rabbit anti-Human Polyclonal (aa651-864) Antibody

Application:

IHC, IHC-P, IHC-Fr, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CD143 antibody LS-C343986 is an unconjugated rabbit polyclonal antibody to CD143 (ACE) (aa651-864) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Human ACE / CD143

Synonyms

See All ACE / CD143 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human ACE recombinant protein (Position: K651-Y864). Human ACE shares 73% and 76% amino acid (aa) sequences identity with mouse and rat ACE, respectively.

Epitope

aa651-864

Specificity

Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .

Applications

IHC
IHC - Paraffin
IHC - Frozen
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ACE / CD143

Uniprot: P12821 NCBI: NM_000789 NP_000780.1