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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B2558-50 50 µg (0.04 mg/ml) $485 
ACE / CD143 Antibody - Anti-ACE antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

PathPlus™ Monoclonal Mouse anti‑Human ACE / CD143 Antibody (IHC, WB) LS‑B2558

PathPlus™ Monoclonal Mouse anti‑Human ACE / CD143 Antibody (IHC, WB) LS‑B2558

Note: This antibody replaces LS-C3696
Antibody:
ACE / CD143 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B2558-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ACE / CD143 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
ACE (Angiotensin-converting enzyme, CD143) is an enzyme involved in catalyzing the conversion of angiotensin I into the physiologically active angiotensin II. It has a central role in the renin-angiotensin system, which is involved in regulating blood pressure and fluid volume in the body. The 287 bp Alu repeat element in this gene is correlated with levels of circulating ACE enzyme and with cardiovascular patho-physiologies. Variants in ACE are also associated with Alzheimer’s disease, where deregulated levels of the protein have been found. In immunohistochemistry, ACE has positive staining in the lung capillary endothelium, basal ganglia neuropil, granulosa cells, Leydig cells, proximal renal tubule brush borders, the uterus, male genitalia, and endothelial cells of small or medium arteries. References: Am J Transl Res. 2011 Jan 1; 3(1): 73–80, PMID: 21139807; Alzheimers Res Ther. 2014; 6(3): 27, PMID: 24987467
Target
Human ACE / CD143
Synonyms
ACE | ACE1 | Angiotensin-converting enzyme | CD143 | CD143 antigen | Carboxycathepsin | DIPEPTIDYL CARBOXYPEPTIDASE | DCP | Dipeptidyl carboxypeptidase I | ICH | Kininase II | MVCD3 | Peptidyl-dipeptidase A | Peptidase P | DCP1 | Dipeptidyl carboxypeptidase 1 | Testicular ECA
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Angiotensin-converting enzyme (denatured) from human kidney
Specificity
Denatured Angiotensin-converting enzyme (ACE). Species cross-reactivity: Human, monkey, rabbit, bovine, canine, feline, guinea pig, rat and mouse.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2558 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2558 was determined to be 10 ug/ml.
Presentation
No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACE / CD143
P12821 NM_000789 NP_000780.1

Validation

ACE / CD143 Antibody - Anti-ACE antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ACE antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ACE antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

PathPlus™ ACE / CD143 Antibody for IHC, WB/Western LS-B2558 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/29/2020