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NOS3 / eNOS

nitric oxide synthase 3 (endothelial cell)

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Gene Name: nitric oxide synthase 3 (endothelial cell)
Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3
Target Sequences: NM_000603 NP_000594.2 P29474

Publications (2)

1
Restoration of perivascular adipose tissue function in diet-induced obese mice without changing bodyweight. Xia N, Weisenburger S, Koch E, Burkart M, Reifenberg G, Förstermann U, Li H. British journal of pharmacology. 2017 174:3443-3453. (WB; Mouse) [Full Text Article] [PubMed:28055105] [PMC:PMC5610154]
2
The Involvement of Sirtuin 1 Dysfunction in High-Fat Diet-Induced Vascular Dysfunction in Mice. Ning Xia, Gisela Reifenberg, Christian Schirra, Huige Li. Antioxidants (Basel, Switzerland). 2022 March;11: [Full Text Article] [PubMed:35326191] [PMC:PMC8944782]

☰ Filters
Antibodies (172)
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NOS3 / eNOS (214)
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NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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NOS3 / eNOS Rabbit anti-Human Polyclonal (aa597-611) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Horse, Bat, Human, Monkey
ICC, IHC, IHC-P, WB
Unconjugated
50 µl/$485
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1186-1203) Antibody
Human
IHC, IHC-P, IP, WB
Unconjugated
1 Each/$485
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1181-1194) Antibody
Rabbit, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50 µg/$645
NOS3 / eNOS Antibody - Immunofluorescent analysis of eNOS (pS1177) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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NOS3 / eNOS Rabbit anti-Human Polyclonal (pSer1177) Antibody
Rabbit, Mouse, Dog, Sheep, Rat, Pig, Human
ICC, IF, IP, WB
Unconjugated
100 µl/$315
NOS3 / eNOS ELISA Kit
Select
Sandwich
96-Well Strip Plate
Mouse
15.625 - 1000 pg/ml
Colorimetric - 450nm (TMB)
Tissue Homogenates
1 Plate/$579
NOS3 / eNOS ELISA Kit
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Cell-Based Phosphorylation
96-Well Microplate
Mouse, Human, Rat
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
1 Plate/$668
NOS3 / eNOS ELISA Kit
Select
Sandwich
96-Well Microplate
Human
62.5 - 4000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$528
NOS3 / eNOS ELISA Kit
Select
Sandwich
96-Well Microplate
Rat
15.63 - 1000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$528
NOS3 / eNOS ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
78 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$585
NOS3 / eNOS ELISA Kit
Select
Sandwich
96-Well Strip Plate
Rat
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$715
NOS3 / eNOS ELISA Kit
Select
Sandwich
96-Well Strip Plate
Mouse
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$782
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NOS3 / eNOS Rabbit anti-Human Polyclonal (Ser1177) (FITC) Antibody
Human
ELISA, Flo, ICC, IF, WB
FITC Conjugated
200 µl/$952
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NOS3 / eNOS Rabbit anti-Bovine Polyclonal (pSer1179) Antibody
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Human
ELISA, IF, WB
Unconjugated
50 µg/$786
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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NOS3 / eNOS Mouse anti-Human Monoclonal (6H2) Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$460
NOS3 / eNOS Antibody - Anti-NOS3 / eNOS antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1181-1194) Antibody
Rabbit, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50 µg/$485
NOS3 / eNOS Antibody - Western blot of recombinant NOS3 / eNOS encoding amino acids 522-705.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NOS3 / eNOS Rabbit anti-Pig Polyclonal (aa522-705) Antibody
Rat, Pig
WB
Unconjugated
100 µl/$317; 200 µl/$362; 500 µl/$513; 1 ml/$589; 2 ml/$812; 5 ml/$1,324
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NOS3 / eNOS Mouse anti-Bovine Monoclonal Antibody
Bovine
ELISA, IP, WB
Unconjugated
100 µg/$786
NOS3 / eNOS Antibody - Detection limit for recombinant GST tagged NOS3 is 3 ng/ml as a capture antibody.
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NOS3 / eNOS Mouse anti-Human Monoclonal (1D12) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$423
NOS3 / eNOS Antibody - Immunohistochemical analysis of eNOS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NOS3 / eNOS Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Rabbit, Mouse, Dog, Sheep, Bovine, Rat, Pig, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$321
NOS3 / eNOS Protein - Recombinant Nitric Oxide Synthase 3, Endothelial By SDS-PAGE
Select
E. coli
His
21.8 kDa
10 µg/$285; 50 µg/$470; 100 µg/$750; 200 µg/$937; 1 mg/$2,063; 500 µg/$1,594; 5 mg/$3,277; 2 mg/$2,252
NOS3 / eNOS Antibody - Confocal immunofluorescence of eNos Antibody (S1177) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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NOS3 / eNOS Rabbit anti-Human Polyclonal (Ser1177) Antibody
Human
Flo, IF, WB
Unconjugated
400 µl/$393
NOS3 / eNOS Antibody - Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.
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NOS3 / eNOS Mouse anti-Human Monoclonal (6H2) Antibody
Human
IHC, IHC-P, WB
Unconjugated
0.1 ml/$386
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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NOS3 / eNOS Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µg/$495
NOS3 / eNOS Antibody - Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NOS3 / eNOS Mouse anti-Human Monoclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100 µl/$321
NOS3 / eNOS Antibody - Immunofluorescence analysis of HeLa cells, using eNOS Antibody. The picture on the right is blocked with the synthesized peptide.
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NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1145-1194) Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
Viewing 1-25 of 214 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).