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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPP1 / Osteopontin Antibody (clone 7C5H12) LS‑C169155
Osteopontin antibody LS-C169155 is an unconjugated mouse monoclonal antibody to human Osteopontin (SPP1). Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C169155-100
100 µg (1 mg/ml)
$365
Description
Osteopontin antibody LS-C169155 is an unconjugated mouse monoclonal antibody to human Osteopontin (SPP1). Validated for ELISA, IF, IHC and WB.
Target
Human SPP1 / Osteopontin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [7C5H12]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of human SPP1 (AA: 167-314) expressed in E. Coli.
Specificity
Human
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
About SPP1 / Osteopontin
P10451 NM_000582 NP_000573.1

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Images

Immunohistochemistry

IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using SPP1 monoclonal antibody against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using SPP1 monoclonal antibody against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot using SPP1 monoclonal antibody against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)
Western blot using SPP1 monoclonal antibody against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using SPP1 monoclonal antibody against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using SPP1 monoclonal antibody against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot using SPP1 monoclonal antibody against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)
Western blot using SPP1 monoclonal antibody against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using SPP1 monoclonal antibody against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using SPP1 monoclonal antibody against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot using SPP1 monoclonal antibody against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)
Western blot using SPP1 monoclonal antibody against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using SPP1 monoclonal antibody against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using SPP1 monoclonal antibody against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot using SPP1 monoclonal antibody against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)
Western blot using SPP1 monoclonal antibody against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: United States
Date Requested: 12/18/2018
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