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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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SMAD1

SMAD family member 1

SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Gene Name: SMAD family member 1
Synonyms: SMAD1, BSP1, BSP-1, JV4-1, MADH1, Mothers against DPP homolog 1, JV41, SMAD family member 1, TGF-beta signaling protein 1, MADR1, HSMAD1, MAD homolog 1, Mad-related protein 1, SMAD 1
Target Sequences: NM_005900 NP_005891.1 Q15797

Publications (4)

1
Axotomy-induced Smad1 activation promotes axonal growth in adult sensory neurons. Zou H, Ho C, Wong K, Tessier-Lavigne M. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2009 29:7116-23. (WB, IP) [PubMed:19494134] [PMC:PMC2739099]
2
Progesterone receptor membrane component-1 regulates hepcidin biosynthesis. Li X, Rhee DK, Malhotra R, Mayeur C, Hurst LA, Ager E, Shelton G, Kramer Y, McCulloh D, Keefe D, Bloch KD, Bloch DB, Peterson RT. The Journal of clinical investigation. 2016 126:389-401. (WB; Human) [Full Text Article] [PubMed:26657863] [PMC:PMC4701562] Related Antibodies: LS-C75853.
3
BMP type I receptor ALK2 is required for angiotensin II-induced cardiac hypertrophy. Shahid M, Spagnolli E, Ernande L, Thoonen R, Kolodziej SA, Leyton PA, Cheng J, Tainsh RE, Mayeur C, Rhee DK, Wu MX, Scherrer-Crosbie M, Buys ES, Zapol WM, Bloch KD, Bloch DB. American journal of physiology. Heart and circulatory physiology. 2016 April;310:H984-H994. [Full Text Article] [PubMed:26873969] [PMC:PMC4867336] Related Antibodies: LS-C75853.
4
The type I BMP receptor Alk3 is required for the induction of hepatic hepcidin gene expression by interleukin-6. Mayeur C, Lohmeyer LK, Leyton P, Kao SM, Pappas AE, Kolodziej SA, Spagnolli E, Yu B, Galdos RL, Yu PB, Peterson RT, Bloch DB, Bloch KD, Steinbicker AU. Blood. Apr;123:2261-8. [Full Text Article] [PubMed:24501215] [PMC:PMC3975261] Related Antibodies: LS-C75853.
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Viewing 1-25 of 154 items
SMAD1 Antibody - Anti-SMAD1 antibody IHC staining of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $425.00
SMAD1 Antibody - Human, Colon: Formalin-Fixed Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Xenopus, Chicken, Sheep, Bovine, Rabbit, Mouse, Guinea pig, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl   $485.00
Select
Mouse Monoclonal (IgG1) to Human SMAD1
Mouse, Rat, Human
Flo, ICC, IP, WB
Unconjugated
50µg   $435.00
SMAD1 Antibody - Immunohistochemical analysis of SMAD1 (pS187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human SMAD1
Chicken, Sheep, Mouse, Rat, Human
IHC, IHC-P, IP, WB
Unconjugated
100µl   1mg/ml   $265.00
SMAD1 Antibody - SMAD1 antibody SMAD1(SMAD family member 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Xenopus, Chicken, Zebrafish, Insect, Drosophila, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl   0.5mg/ml   $325.00
SMAD1 Antibody - Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
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Mouse Monoclonal [clone 1D3] (IgG1,k) to Human SMAD1
Human
ELISA, IF, WB
Unconjugated
100µg   $365.00
SMAD1 Antibody - Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
Select
Mouse Monoclonal [clone 2E9] (IgG1,k) to Human SMAD1
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
100µg   $365.00
SMAD1 Antibody - Immunofluorescent staining using SMAD1 antibody. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-SMAD1 antibody.
Select
Mouse Monoclonal [clone 913C1b] (IgG1) to Human SMAD1
Mouse, Rat, Human
DB, Flo, ICC, IP, WB
Unconjugated
50µg   100µg/ml   $315.00
SMAD1 Antibody - Western blot of Phospho-Smad1 (S465) antibody
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
SMAD1 Antibody - Immunohistochemistry (IHC) analysis of p-Smad1 (S465) pAb in paraffin-embedded human heart tissue.
Select
Rabbit Polyclonal to Human SMAD1
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl   $345.00
SMAD1 Antibody - Anti-SMAD1 Antibody - Western Blot. Western blot of Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37?C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n LS-B323) staining was used as a loading control. The membrane was washed and reacted with a 1:3000 dilution HRP-conjugated a-Rabbit IgG (p/n LS-C60865) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Mouse, Dog, Rat, Human
ELISA, IP, WB
Unconjugated
100µg   1.31mg/ml   $425.00
SMAD1 Antibody - Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SMAD1 mAb.
Select
Mouse Monoclonal [clone 913C1b] (IgG1) to Human SMAD1
Mouse, Rat, Human
DB, Flo, ICC, IF, IP, WB
Unconjugated
100µg   100µg/ml   $415.00
SMAD1 Antibody - 1:100 staining mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Monoclonal [clone D40B7] (IgG) to Human SMAD1
Mouse, Rat, Human, Monkey
IP, WB
Unconjugated
20µl   $405.00; 100µl   $650.00
SMAD1 Antibody - 1:100 staining mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Mouse, Rat, Human, Monkey
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
SMAD1 Antibody - 1:100 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
SMAD1 Antibody - SMAD1 antibody. IHC(P): Human Lung Cancer Tissue.
Select
Rabbit Polyclonal to Mouse SMAD1
Mouse, Human
ICC, IHC, IHC-P, WB
Unconjugated
100µg   0.5mg/ml   $355.00
SMAD1 Antibody - A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $335.00
SMAD1 Antibody - Immunohistochemical of paraffin-embedded H. heart section using SMAD1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Select
Rabbit Polyclonal to Human SMAD1
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
400µl   $325.00
SMAD1 Antibody - SMAD1 Antibody staining SMAD1 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Select
Mouse Monoclonal [clone 1356CT119.18.55] (IgG1,k) to Human SMAD1
Human
ELISA, IF, IHC-P, WB
Unconjugated
200µl   $325.00
SMAD1 Antibody - Immunohistochemistry of paraffin-embedded human heart tissue at dilution 1:100
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50µg   $285.00; 100µg   $385.00
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Human
ELISA, IF, IHC, WB
Unconjugated
100µg   $355.00
SMAD1 Antibody - Western blot of Smad1 antibody
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
SMAD1 Antibody - Immunohistochemistry of paraffin-embedded human stomach cancer tissue.
Select
Rabbit Polyclonal (IgG) to Human SMAD1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $315.00; 200µl   $425.00
Select
Rabbit Polyclonal to Human SMAD1
Mouse, Rat, Human
ICC, IHC, IP, WB
Unconjugated
100µl   $345.00

Viewing 1-25 of 154 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).