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Antibodies > SMAD1 > LS-C757687
 
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Catalog Number Size Price
LS-C757687-200 200 µl $393 
SMAD1 Antibody - SMAD1 Antibody staining SMAD1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
SMAD1 Antibody - SMAD1 Antibody staining SMAD1 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
SMAD1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling SMAD1 with SMAD1 Antibody at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NH174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
SMAD1 Antibody - All lanes: Anti-SMAD1 Antibody at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
SMAD1 Antibody - SMAD1 Antibody staining SMAD1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
SMAD1 Antibody - SMAD1 Antibody staining SMAD1 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
SMAD1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling SMAD1 with SMAD1 Antibody at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NH174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
SMAD1 Antibody - All lanes: Anti-SMAD1 Antibody at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Monoclonal Mouse anti‑Human SMAD1 Antibody (clone 1356CT119.18.55, IHC, IF, WB) LS‑C757687

Monoclonal Mouse anti‑Human SMAD1 Antibody (clone 1356CT119.18.55, IHC, IF, WB) LS‑C757687

Submit Review LSBio Guarantee
Antibody:
SMAD1 Mouse anti-Human Monoclonal (1356CT119.18.55) Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757687-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SMAD1 Mouse anti-Human Monoclonal (1356CT119.18.55) Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD1 antibody LS-C757687 is an unconjugated mouse monoclonal antibody to human SMAD1. Validated for ELISA, IF, IHC and WB.
Target
Human SMAD1
Synonyms
SMAD1 | BSP1 | BSP-1 | JV4-1 | MADH1 | Mothers against DPP homolog 1 | JV41 | SMAD family member 1 | TGF-beta signaling protein 1 | MADR1 | HSMAD1 | MAD homolog 1 | Mad-related protein 1 | SMAD 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1356CT119.18.55
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
This SMAD1 antibody is generated from a mouse immunized with a recombinant protein between 20-330 amino acids from human SMAD1.
Applications
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 52.3 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD1
Q15797 NM_005900 NP_005891.1

Publications (0)

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Featured Products

Product
SMAD1 Antibody LS‑C75853
Species: Human, Mouse, Rat
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry
SMAD1 Antibody - Anti-SMAD1 Antibody - Western Blot. Western blot of Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37?C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n LS-B323) staining was used as a loading control. The membrane was washed and reacted with a 1:3000 dilution HRP-conjugated a-Rabbit IgG (p/n LS-C60865) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.
SMAD1 Antibody LS‑C154249
Species: Human, Mouse, Rat, Dog
Applications: Western blot, Immunoprecipitation, ELISA
SMAD1 Antibody - Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
SMAD1 Antibody (clone 1D3) LS‑C133298
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
SMAD1 Antibody - SMAD1 antibody SMAD1(SMAD family member 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SMAD1 Antibody (N‑Terminus) LS‑C80224
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
SMAD1 Antibody - A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SMAD1 Antibody (C‑Terminus) LS‑C31108
Species: Human
Applications: Western blot
SMAD1 Antibody - Immunohistochemical analysis of SMAD1 (pS187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SMAD1 Antibody (phospho‑Ser187) LS‑C352485
Species: Human, Mouse, Rat, Sheep, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
See all SMAD1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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