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| Catalog Number | Size | Price |
|---|---|---|
| LS-C75853-50 | 50 µg (0.1 mg/ml) | $558 |
Monoclonal Mouse anti‑Human SMAD1 Antibody (WB) LS‑C75853
Monoclonal Mouse anti‑Human SMAD1 Antibody (WB) LS‑C75853
Antibody:
SMAD1 Mouse anti-Human Monoclonal Antibody
Application:
ICC, WB, IP, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
SMAD1 Mouse anti-Human Monoclonal Antibody
Application:
ICC, WB, IP, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
SMAD1 antibody LS-C75853 is an unconjugated mouse monoclonal antibody to SMAD1 from human. It is reactive with human, mouse and rat. Validated for Flow, ICC, IP and WB. Cited in 4 publications.
Target
Human SMAD1
Synonyms
SMAD1 | BSP1 | BSP-1 | JV4-1 | MADH1 | Mothers against DPP homolog 1 | JV41 | SMAD family member 1 | TGF-beta signaling protein 1 | MADR1 | HSMAD1 | MAD homolog 1 | Mad-related protein 1 | SMAD 1
Host
Mouse
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human Smad1.
Specificity
Recognizes human Smad1.
Applications
- ICC (2 - 100 µg/ml)
- Western blot (0.2 - 2 µg/ml)
- Immunoprecipitation
- Flow Cytometry (0.5 - 2 µg/ml)
Failed Applications
- IHC - Paraffin
Usage
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Flow Cytometry: 0.5-2 ug/ml. HeLa cells were fixed with 2% paraformaldehyde in 90% methanol. Western Blot: 0.2-2 ug/ml. Immunocytochemistry: 2-100 ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Immunoprecipitation: 100-500 ug/sample.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 1% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SMAD1
LSBio Ratings
SMAD1 Antibody for ICC, WB/Western, IP, Flow LS-C75853 has an LSBio Rating of
Publications (4.3)
Learn more about The LSBio Ratings Algorithm
Publications (4)
The type I BMP receptor Alk3 is required for the induction of hepatic hepcidin gene expression by interleukin-6. Mayeur C, Lohmeyer LK, Leyton P, Kao SM, Pappas AE, Kolodziej SA, Spagnolli E, Yu B, Galdos RL, Yu PB, Peterson RT, Bloch DB, Bloch KD, Steinbicker AU. Blood. Apr;123:2261-8.
Progesterone receptor membrane component-1 regulates hepcidin biosynthesis. Li X, Rhee DK, Malhotra R, Mayeur C, Hurst LA, Ager E, Shelton G, Kramer Y, McCulloh D, Keefe D, Bloch KD, Bloch DB, Peterson RT. The Journal of clinical investigation. 2016 126:389-401. (WB; Human)
BMP type I receptor ALK2 is required for angiotensin II-induced cardiac hypertrophy. Shahid M, Spagnolli E, Ernande L, Thoonen R, Kolodziej SA, Leyton PA, Cheng J, Tainsh RE, Mayeur C, Rhee DK, Wu MX, Scherrer-Crosbie M, Buys ES, Zapol WM, Bloch KD, Bloch DB. American journal of physiology. Heart and circulatory physiology. 2016 April;310:H984-H994.
Transforming growth factor-ß stimulates Smad1/5 signaling in pulmonary artery smooth muscle cells and fibroblasts of the newborn mouse through ALK1. Huili Zhang, Lili Du, Ying Zhong, Kathleen C Flanders, Jesse D Roberts Jr. American journal of physiology. Lung cellular and molecular physiology. 2017 September;313:L615-L627.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Dog
Applications:
Western blot, Immunoprecipitation, ELISA
![SMAD1 Antibody - Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/smad1-antibody-clone-1d3-ls-c133298/51116_1275040.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Sheep, Chicken
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Reactivity:
Human, Mouse, Rat
Range:
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/22/2024
Date Requested: 4/22/2024
