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RAF1 / RAF

v-raf-1 murine leukemia viral oncogene homolog 1

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Gene Name: v-raf-1 murine leukemia viral oncogene homolog 1
Family/Subfamily: Protein Kinase , Raf
Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase
Target Sequences: NM_002880 NP_002871.1 P04049

Publications (1)

1
PTPIP51, a novel 14-3-3 binding protein, regulates cell morphology and motility via Raf-ERK pathway. Yu C, Han W, Shi T, Lv B, He Q, Zhang Y, Li T, Zhang Y, Song Q, Wang L, Ma D. Cellular signalling. 2008 20:2208-20. (IP) [PubMed:18771726]

☰ Filters
Antibodies (241)
ELISA Kits (13)
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Primary (241)
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RAF1 / RAF (262)
Human (247)
Mouse (118)
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aa256-286 (6)
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aa1-648 (4)
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200-280 aa (2)
240-320 aa (2)
280-360 aa (2)
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Human (7)
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Purified (1)
RAF1 / RAF Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
RAF1 / RAF Rabbit anti-Mouse Polyclonal (aa86-113) Antibody
Mouse, Human
Flo, IHC, IHC-P, WB
Unconjugated
200 µl/$460
RAF1 / RAF Antibody - Anti-RAF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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RAF1 / RAF Mouse anti-Human Monoclonal (N-Terminus) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
RAF1 / RAF Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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RAF1 / RAF Rabbit anti-Human Polyclonal (aa1-30) Antibody
Human
Flo, IF, IHC, WB
Unconjugated
400 µl/$393
RAF1 / RAF Protein - 12.5% SDS-PAGE of human RAF1 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
99.5 kDa
2 µg/$439
RAF1 / RAF Protein
Select
E. coli
His-SUMO, N-terminus
89 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
RAF1 / RAF Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
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RAF1 / RAF Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Chicken, Bovine, Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$460
RAF1 / RAF Antibody - IHC of paraffin-embedded liver cancer tissues using RAF1 mouse monoclonal antibody with DAB staining.
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RAF1 / RAF Mouse anti-Human Monoclonal (4G4) Antibody
Human
ELISA, Flo, IHC, IHC-P, WB
Unconjugated
100 µl/$459
RAF1 / RAF Protein - Purified recombinant protein RAF1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
72.9 kDa
20 µg/$1,107
RAF1 / RAF Antibody - 1:200 staining human lymph node tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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RAF1 / RAF Rabbit anti-Human Polyclonal (pSer621) Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
RAF1 / RAF ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Tissue Homogenates
1 Plate/$730
RAF1 / RAF Protein - Recombinant C-Raf Proto Oncogene Serine/Threonine Protein Kinase By SDS-PAGE
Select
E. coli
His-T7
35.6 kDa
10 µg/$271; 50 µg/$382; 100 µg/$610; 200 µg/$762; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
RAF1 / RAF Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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RAF1 / RAF Rabbit anti-Human Polyclonal (pSer338) Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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RAF1 / RAF Rabbit anti-Human Polyclonal (aa256-286) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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RAF1 / RAF Rabbit anti-Human Polyclonal (Pro302) Antibody
Human, Monkey
IP, WB
Unconjugated
100 µl/$688
RAF1 / RAF Antibody - Western blot of Phospho-RAF1-pS338 Antibody in human TPA activated HeLa cell line lysates. Phospho-RAF1 (arrow) was detected using the purified antibody. (0: without TPA; 1: 60 ug/ml TPA, 15min; 2: 60 ug/ml TPA, 30min; 3: 60 ug/ml TPA, 45min; 4: 125 ug/ml TPA, 15min; 5: 125 ug/ml TPA, 30min; 6: 125 ug/ml TPA, 45min; 7: 250 ug/ml TPA, 15min; 8: 250 ug/ml TPA, 30min; 9: 250 ug/ml, 45min)
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RAF1 / RAF Rabbit anti-Human Polyclonal (pSer338) Antibody
Human
DB, WB
Unconjugated
400 µl/$393
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RAF1 / RAF Sheep anti-Human Polyclonal (aa657-668) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Horse, Bat, Human, Monkey
IP, WB
Unconjugated
100 µg/$567
RAF1 / RAF Antibody - IHC of paraffin-embedded bladder cancer tissues using RAF1 mouse monoclonal antibody with DAB staining.
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RAF1 / RAF Mouse anti-Human Monoclonal (4G4) Antibody
Human
ELISA, Flo, IHC, WB
Unconjugated
100 µl/$459
RAF1 / RAF Antibody - Western blot of recombinant RAF1 / RAF.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAF1 / RAF Rabbit anti-Human Polyclonal (aa342-619) Antibody
Human
WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
RAF1 / RAF Antibody - P-peptide - + Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Raf1 (Phospho-Ser621) antibody.
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RAF1 / RAF Rabbit anti-Human Polyclonal (pSer621) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$357
RAF1 / RAF Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Raf1 (Ab-621) antibody.
Select
RAF1 / RAF Rabbit anti-Human Polyclonal (p621) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$357
Select
RAF1 / RAF Rabbit anti-Human Monoclonal (pSer338) Antibody
Mouse, Rat, Human, Monkey
WB
Unconjugated
100 µl/$689
RAF1 / RAF Antibody - 1:100 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
RAF1 / RAF Rabbit anti-Human Polyclonal (pSer296) Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
RAF1 / RAF Antibody - 1:200 staining human colon carcinoma sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22° C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
RAF1 / RAF Rabbit anti-Human Polyclonal (pSer259) Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
RAF1 / RAF Antibody - Western blot of C-RAF phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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RAF1 / RAF Rabbit anti-Human Polyclonal (pSer621) Antibody
Mouse, Rat, Human
ICC, IF, IHC, WB
Unconjugated
50 µg/$305; 100 µg/$357
RAF1 / RAF Antibody - Western blot of C-RAF expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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RAF1 / RAF Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ICC, IF, IHC, WB
Unconjugated
50 µg/$305; 100 µg/$357
Viewing 1-25 of 262 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).