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Catalog Number Size Price
LS-C9887-100 100 µg $567 
Antibody

Polyclonal Sheep anti‑Human RAF1 / RAF Antibody (aa657‑668, WB) LS‑C9887

Polyclonal Sheep anti‑Human RAF1 / RAF Antibody (aa657‑668, WB) LS‑C9887

Antibody:
RAF1 / RAF Sheep anti-Human Polyclonal (aa657-668) Antibody
Application:
WB, IP
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$567
LS-C9887-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RAF1 / RAF Sheep anti-Human Polyclonal (aa657-668) Antibody
Application:
WB, IP
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
RAF antibody LS-C9887 is an unconjugated sheep polyclonal antibody to RAF (RAF1) (aa657-668) from human. It is reactive with human, mouse, rat and other species. Validated for IP and WB.
Target
Human RAF1 / RAF
Synonyms
RAF1 | C-Raf | C-raf-1 | CRAF | NS5 | Raf-1 | Proto-oncogene c-RAF | Oncogene RAF1 | RAF | Raf1 kinase
Host
Sheep
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
12 residue synthetic C-Terminus peptide (CTLTTSPRLPVF) corresponding to aa 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
Epitope
aa657-668
Specificity
Recognizes Raf-1, Mr ~70kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
Applications
  • Western blot
  • Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 ug/ml detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 ug immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAF1 / RAF
P04049 NM_002880 NP_002871.1

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Requested From: United States
Date Requested: 2/25/2024