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Catalog Number Size Price
LS-B14835-50 50 µl (1 mg/ml) $460 
RAF1 / RAF Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAF1 / RAF Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAF1 / RAF Antibody - Immunohistochemical analysis of c-RAF staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
RAF1 / RAF Antibody - Immunofluorescent analysis of c-RAF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
RAF1 / RAF Antibody - Western blot analysis of c-RAF expression in HeLa (A); NIH3T3 (B) whole cell lysates.
RAF1 / RAF Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAF1 / RAF Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAF1 / RAF Antibody - Immunohistochemical analysis of c-RAF staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
RAF1 / RAF Antibody - Immunofluorescent analysis of c-RAF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
RAF1 / RAF Antibody - Western blot analysis of c-RAF expression in HeLa (A); NIH3T3 (B) whole cell lysates.
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IHC‑plus™ Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (C‑Terminus, IHC, IF, WB) LS‑B14835

IHC‑plus™ Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (C‑Terminus, IHC, IF, WB) LS‑B14835

Note: This antibody replaces LS-C413188
Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14835-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
RAF antibody LS-B14835 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RAF1 / RAF
Synonyms
RAF1 | C-Raf | C-raf-1 | CRAF | NS5 | Raf-1 | Proto-oncogene c-RAF | Oncogene RAF1 | RAF | Raf1 kinase
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human c-RAF.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of c-RAF protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAF1 / RAF
P04049 NM_002880 NP_002871.1

Validation

RAF1 / RAF Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

RAF1 / RAF Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ RAF1 / RAF Antibody (C-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B14835 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/25/2024