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NXF / NPAS4

neuronal PAS domain protein 4

Acts as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Gene Name: neuronal PAS domain protein 4
Synonyms: NPAS4, BHLHe79, Neuronal PAS domain protein 4, Neuronal PAS4, Le-PAS, NXF, PASD10
Target Sequences: NP_849195.2 Q8IUM7

Filtered by: None
Target
NXF / NPAS4 (33)
Reactivity
Human (32)
Mouse (27)
Rat (25)
Bat (1)
Bovine (5)
Chimpanzee (3)
Dog (5)
Gibbon (3)
Hamster (1)
Horse (5)
Monkey (6)
Pig (6)
Rabbit (5)
Application
IHC (22)
IHC-P (2)
WB (27)
ELISA (3)
ICC (1)
IF (19)
Peptide-ELISA (2)
Host
rabbit (14)
mouse (18)
goat (1)
Isotype
IgG (7)
IgG1 (18)
Clonality
monoclonal mc (18)
polyclonal pc (15)
Clone
S408-79 (18)
Format
AP Conjugated (1)
APC Conjugated (1)
Atto 390 Conjugated (1)
Atto 488 Conjugated (1)
Atto 565 Conjugated (1)
Atto 594 Conjugated (2)
Atto 633 Conjugated (1)
Atto 655 Conjugated (1)
Atto 680 Conjugated (1)
Atto 700 Conjugated (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE (1)
PerCP Conjugated (1)
RPE Conjugated (1)
SA Conjugated (1)
Unconjugated (10)
Epitope
aa539-588 (4)
C-Terminus (2)
580-660 aa, C-terminal (1)
aa149-161 (1)
aa603-652 (1)
Publications
No (33)
Showing 1-25 of 33 results

NXF / NPAS4 Antibody (C-Terminus) LS-C356222
Rabbit Polyclonal to Human NXF / NPAS4
Pig, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of NPAS4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of NPAS4 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

NXF / NPAS4 Antibody (aa603-652) LS-C120156
Rabbit Polyclonal (IgG) to Human NXF / NPAS4
Mouse, Rat, Human
IHC, Peptide-ELISA
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NPAS4 Antibody. The picture on the right is blocked with the synthesized peptide.

NXF / NPAS4 Antibody (aa539-588) LS-C109685
Rabbit Polyclonal to Human NXF / NPAS4
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $385.00
NPAS4 antibody Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

NXF / NPAS4 Antibody LS-C674585
Rabbit Polyclonal (IgG) to Human NXF / NPAS4
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

NXF / NPAS4 Antibody (clone S408-79) LS-C777011
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
100µg 1mg/ml  $365.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, HRP) LS-C777199
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
HRP Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Biotin) LS-C776241
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Biotin Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, FITC) LS-C776243
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
FITC Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, RPE) LS-C774332
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
RPE Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, SA) LS-C774331
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
SA Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, PerCP) LS-C774334
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
PerCP Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Atto 390) LS-C774335
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 390 Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Atto 655) LS-C774337
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 655 Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, APC) LS-C774333
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
APC Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Atto 565) LS-C774336
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 565 Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Atto 633) LS-C776242
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 633 Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, PE, Atto 594) LS-C776244
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
PE, Atto 594 Conjugated
100µg 1mg/ml  $405.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Atto 700) LS-C776246
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 700 Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Atto 680) LS-C776245
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 680 Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, AP) LS-C777198
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
AP Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Atto 488) LS-C777200
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 488 Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (clone S408-79, Atto 594) LS-C777201
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 594 Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

NXF / NPAS4 Antibody (580-660 aa, C-terminal) LS-C384949
Rabbit Polyclonal (IgG) to Human NXF / NPAS4
Mouse, Rat, Human
ELISA, IHC
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

NXF / NPAS4 Antibody (C-Terminus) LS-C144924
Rabbit Polyclonal to Mouse NXF / NPAS4
Mouse
WB
Unconjugated
100µl  $365.00

NXF / NPAS4 Antibody (aa149-161) LS-C139716
Goat Polyclonal to Human NXF / NPAS4
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
NPAS4 antibody (0.3 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NPAS4 Antibody (1µg/ml) staining of Mouse (A) and Rat (B) Brain lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).