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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NXF / NPAS4

neuronal PAS domain protein 4

Acts as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Gene Name: neuronal PAS domain protein 4
Synonyms: NPAS4, BHLHe79, Neuronal PAS domain protein 4, Neuronal PAS4, Le-PAS, NXF, PASD10
Target Sequences: NP_849195.2 Q8IUM7


NXF / NPAS4 Antibody - Immunohistochemical analysis of NPAS4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human NXF / NPAS4
Pig, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
NXF / NPAS4 Antibody - Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NPAS4 Antibody. The picture on the right is blocked with the synthesized peptide.
Select
Rabbit Polyclonal (IgG) to Human NXF / NPAS4
Mouse, Rat, Human
IHC, Peptide-ELISA
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
NXF / NPAS4 Antibody - NPAS4 antibody Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human NXF / NPAS4
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $385.00
NXF / NPAS4 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
Rabbit Polyclonal (IgG) to Human NXF / NPAS4
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
100µg   1mg/ml   $365.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Biotin Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
FITC Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
SA Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
RPE Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 655 Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 390 Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
PerCP Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
APC Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 565 Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 633 Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
PE, Atto 594 Conjugated
100µg   1mg/ml   $405.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 680 Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 700 Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
AP Conjugated
100µg   1mg/ml   $395.00
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Select
Mouse Monoclonal [clone S408-79] (IgG1) to Human NXF / NPAS4
Mouse, Rat, Human
IF, IHC, WB
Atto 594 Conjugated
100µg   1mg/ml   $395.00
Select
Rabbit Polyclonal (IgG) to Human NXF / NPAS4
Mouse, Rat, Human
ELISA, IHC
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
NXF / NPAS4 Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using NPAS4 antibody.
Select
Rabbit Polyclonal (IgG) to Human NXF / NPAS4
Mouse, Rat, Human
ELISA, IHC
Unconjugated
100µg   $305.00
Select
Rabbit Polyclonal to Mouse NXF / NPAS4
Mouse
WB
Unconjugated
100µl   $285.00; 200µl   $345.00
NXF / NPAS4 Antibody - NPAS4 antibody (0.3 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Select
Goat Polyclonal to Human NXF / NPAS4
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100µg   0.5mg/ml   $405.00
NXF / NPAS4 Antibody - Western blot of NPAS4 antibody at 1:500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: RAW264.7 whole cell lysate.
Select
Rabbit Polyclonal to Human NXF / NPAS4
Mouse, Human
WB
Unconjugated
100µl   1mg/ml   $385.00
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).