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Catalog Number Size Price
LS-C774332-100 100 µg (1 mg/ml) $477 
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
NXF / NPAS4 Antibody - Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.
NXF / NPAS4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
NXF / NPAS4 Antibody - Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.
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Monoclonal Mouse anti‑Human NXF / NPAS4 Antibody (clone S408‑79, RPE, IHC, IF, WB) LS‑C774332

Monoclonal Mouse anti‑Human NXF / NPAS4 Antibody (clone S408‑79, RPE, IHC, IF, WB) LS‑C774332

Antibody:
NXF / NPAS4 Mouse anti-Human Monoclonal (RPE) (S408-79) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Other formats:
Price
Catalog Number
$477
LS-C774332-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NXF / NPAS4 Mouse anti-Human Monoclonal (RPE) (S408-79) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Other formats:

Specifications

Description
NPAS4 antibody LS-C774332 is an RPE-conjugated mouse monoclonal antibody to NPAS4 (NXF) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human NXF / NPAS4
Synonyms
NPAS4 | BHLHe79 | Neuronal PAS domain protein 4 | Neuronal PAS4 | Le-PAS | NXF | PASD10
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
S408-79
Conjugations
R. Phycoerythrin. Also available conjugated with APC, Atto 390, Atto 594, Biotin, FITC, PerCP.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Fusion protein amino acids 597-802 (C-Terminus) of rat Npas4.
Specificity
Detects ~90 kDa.
Applications
  • IHC
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NXF / NPAS4
Q8IUM7 NP_849195.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024