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NXF / NPAS4 Antibody (aa539‑588) LS‑C109685

Catalog Number Size Price
LS-C109685-100 100 µl (0.5 mg/ml) $385 
NPAS4 antibody Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

NXF / NPAS4 Antibody (aa539‑588) LS‑C109685

Antibody:
Rabbit Polyclonal to Human NXF / NPAS4
Application:
WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$385 
LS-C109685-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NXF / NPAS4
Application:
WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NPAS4 antibody LS-C109685 is an unconjugated rabbit polyclonal antibody to NPAS4 (NXF ) from human, mouse, rat and other species. Validated for WB.
Target
Human NXF / NPAS4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa539-588 of human NPAS4 (Q8IUM7, NP_849195). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Rabbit, Pig (100%); Elephant, Bat, Guinea pig (92%); Opossum (91%); Bovine, Horse (85%).
Blocking Peptide
LS-E11425 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109685. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa539-588
Specificity
Human NPAS4
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NXF / NPAS4
Q8IUM7 NP_849195.2

Publications (0)

Reviews (0)

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Immunohistochemical analysis of NPAS4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NPAS4 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity: Mouse
Range: 78-5000 pg/ml
Reactivity: Human
Range: 0.312-20 ng/ml
Anti-Factor I antibody IHC of human colon, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Requested From: United States
Date Requested: 9/18/2019