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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP3K5 / ASK1

mitogen-activated protein kinase kinase kinase 5

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Gene Name: mitogen-activated protein kinase kinase kinase 5
Family/Subfamily: Protein Kinase , MAP3K
Synonyms: MAP3K5, ASK-1, ASK1, MEKK5, MEK kinase 5, MEKK 5, MAPK/ERK kinase kinase 5, MAPKKK5, MAP/ERK kinase kinase 5
Target Sequences: NM_005923 NP_005914.1 Q99683

Publications (1)

1
Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase. Subramanyam M, Takahashi N, Hasegawa Y, Mohri T, Okada Y. The Journal of biological chemistry. 2010 285:6109-17. [PubMed:20048146] [PMC:PMC2825405] Related Antibodies: LS-C18846.

Filtered by: None
Target
MAP3K5 / ASK1 (113)
Reactivity
Human (109)
Mouse (46)
Rat (21)
Bovine (2)
Application
IHC (56)
IHC-Fr (2)
IHC-P (19)
WB (103)
Flo (3)
ELISA (42)
ICC (4)
IF (10)
IP (2)
Peptide-ELISA (4)
Host
rabbit (109)
mouse (4)
Isotype
IgG (69)
IgG1 (3)
IgG2b,k (1)
Clonality
monoclonal mc (4)
polyclonal pc (109)
Clone
20000 (1)
2E4 (1)
5C4 (1)
Format
AP Conjugated (6)
APC (2)
APC Conjugated (5)
Azide-free (2)
Biotin Conjugated (7)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (7)
HRP Conjugated (7)
PE Conjugated (6)
Unconjugated (71)
Epitope
Ser966 (8)
aa1175-1203 (8)
aa681-936 (8)
aa693-933 (8)
pSer83 (8)
pSer966 (7)
N-Terminus (3)
20-100 aa (2)
910-990 aa (2)
pSer1033 (2)
pThr918 (2)
C-Terminus (1)
Glu960 (1)
Internal (1)
Val972 (1)
aa1198-1216 (1)
aa280 (1)
aa49-98 (1)
aa932-981 (1)
pSer1046 (1)
Publications
No (112)
Yes (1)
Showing 1-25 of 113 results

IHC-plus™ MAP3K5 / ASK1 Antibody LS-B3205
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-MAP3K5 / ASK1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MAP3K5 / ASK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ MAP3K5 / ASK1 Antibody LS-B3206
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $545.00
Anti-MAP3K5 / ASK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MAP3K5 / ASK1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

MAP3K5 / ASK1 Antibody (phospho-Ser83) LS-C18846
Publications (1)
Publications (1)
Rabbit Polyclonal to Human MAP3K5 / ASK1
Human
ELISA, WB
Unconjugated
200µg 1mg/ml  $445.00
Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.
Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.

MAP3K5 / ASK1 Antibody (20-100 aa) LS-C382174
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of ASK 1 antibody
Western blot of ASK 1 antibody

MAP3K5 / ASK1 Antibody (N-Terminus) LS-C368417
Rabbit Polyclonal to Human MAP3K5 / ASK1
Mouse, Bovine, Rat, Human
IHC, IHC-P, WB
Unconjugated
30µl 1mg/ml  $215.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $325.00
Immunohistochemical analysis of ASK1 (pS83) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of ASK1 (pS83) expression in K562 (A); HeLa (B); SP20 (C); PC12 (D) whole cell lysates.

MAP3K5 / ASK1 Antibody (clone 5C4) LS-C197672
Mouse Monoclonal [clone 5C4] (IgG2b,k) to Human MAP3K5 / ASK1
Human
ELISA, WB
Unconjugated
100µg  $365.00
MAP3K5 monoclonal antibody (M01), clone 5C4 Western Blot analysis of MAP3K5 expression in HepG2.

MAP3K5 / ASK1 Antibody (phospho-Ser966) LS-C177874
Rabbit Polyclonal to Human MAP3K5 / ASK1
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of p-ASK1 (S966)pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of p-ASK1 (S966) pAb in paraffin-embedded human breast carcinoma tissue.

MAP3K5 / ASK1 Antibody (clone 20000) LS-C758667
Mouse Monoclonal [clone 20000] (IgG1) to Human MAP3K5 / ASK1
Human
Flo, ICC, IF, IHC, WB
Unconjugated
100µl  $405.00
ASK1 Antibody in Immunohistochemistry (IHC)
ASK1 Antibody in Immunofluorescence (IF)

MAP3K5 / ASK1 Antibody (clone 2E4) LS-C169375
Mouse Monoclonal [clone 2E4] (IgG1) to Human MAP3K5 / ASK1
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
100µl  $365.00
IHC of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse monoclonal antibody with DAB staining.
Immunofluorescence of HeLa cells using MAP3K5 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

MAP3K5 / ASK1 Antibody LS-C801987
Mouse Monoclonal (IgG1) to Human MAP3K5 / ASK1
Human
ELISA, Flo, ICC, IHC, WB
Unconjugated
50µl 1mg/ml  $315.00; 100µl 1mg/ml  $375.00; 200µl 1mg/ml  $435.00

MAP3K5 / ASK1 Antibody (phospho-Ser966) LS-C801226
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

IHC-plus™ MAP3K5 / ASK1 Antibody (aa49-98) LS-B7565
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-MAP3K5 / ASK1 antibody IHC of human skin, sweat ducts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-MAP3K5 / ASK1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

MAP3K5 / ASK1 Antibody (phospho-Ser83) LS-C801224
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

MAP3K5 / ASK1 Antibody LS-C801664
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

MAP3K5 / ASK1 Antibody LS-C271
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, ICC, IF, WB
Unconjugated
100µg  $365.00
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 µg/ml.
Immunofluorescence of ASK1 in A431 cells with ASK1 antibody at 20 ug/ml.

MAP3K5 / ASK1 Antibody LS-C499284
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IF, IHC
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human testis tissue using MAP3K5 Antibody at dilution of 1:100
Immunofluorescent analysis of Hela cells using MAP3K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

MAP3K5 / ASK1 Antibody (aa1175-1203) LS-C164455
Rabbit Polyclonal to Mouse MAP3K5 / ASK1
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Mouse Map3k5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Mouse Map3k5 Antibody western blot of mouse bladder tissue lysates (35 ug/lane). The Map3k5 antibody detected the Map3k5 protein (arrow).

MAP3K5 / ASK1 Antibody (aa1175-1203) LS-C329813
Rabbit Polyclonal (IgG) to Mouse MAP3K5 / ASK1
Mouse, Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

MAP3K5 / ASK1 Antibody LS-C334609
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of various cell lines.

MAP3K5 / ASK1 Antibody (phospho-Ser83) LS-C117292
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ASK1 (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from MDA-MB-435 cells treated with TNF-alpha, using ASK1 (Phospho-Ser83) Antibody. The lane on the left is blocked with the phospho peptide.

MAP3K5 / ASK1 Antibody (phospho-Ser966) LS-C117293
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ASK1 (Phospho-Ser966) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with TNF(20ng/ml)+calyculinA(50nM) 15', using ASK1 (Phospho-Ser966) Antibody. The lane on the right is blocked with the phospho peptide.

MAP3K5 / ASK1 Antibody (aa1198-1216) LS-C148204
Rabbit Polyclonal to Human MAP3K5 / ASK1
Human
IHC, IHC-Fr, IHC-P, IP, WB
Unconjugated
50µl  $335.00
Formalin-fixed paraffin-embedded tissue section of human reactive tonsil stained for ASK1 expression using Polyclonal Antibody to ASK1 at 1:2000. A and A1. Low and high magnification, respectively. ASK1 expression was seen in the vasculature (endothelial cells). Hematoxylin-Eosin counterstain.
Formalin-fixed paraffin-embedded tissue section of human small venous vessels stained for ASK1 expression using Polyclonal Antibody to ASK1 at 1:2000. ASK1 positive epithelial cells were seen. Hematoxylin-Eos in counterstain.

MAP3K5 / ASK1 Antibody LS-C122698
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IHC, WB
Unconjugated
50µg  $435.00

MAP3K5 / ASK1 Antibody LS-C122699
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µg  $435.00

MAP3K5 / ASK1 Antibody LS-C658600
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IHC, WB
Unconjugated
100µl  $445.00
Showing 1-25 of 113 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).