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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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MAP3K5 / ASK1

mitogen-activated protein kinase kinase kinase 5

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Gene Name: mitogen-activated protein kinase kinase kinase 5
Family/Subfamily: Protein Kinase , MAP3K
Synonyms: MAP3K5, ASK-1, ASK1, MEKK5, MEK kinase 5, MEKK 5, MAPK/ERK kinase kinase 5, MAPKKK5, MAP/ERK kinase kinase 5
Target Sequences: NM_005923 NP_005914.1 Q99683

Publications (1)

1
Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase. Subramanyam M, Takahashi N, Hasegawa Y, Mohri T, Okada Y. The Journal of biological chemistry. 2010 285:6109-17. [PubMed:20048146] [PMC:PMC2825405] Related Antibodies: LS-C18846.


MAP3K5 / ASK1 Antibody - Anti-MAP3K5 / ASK1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
0.05mg   1mg/ml   $425.00
MAP3K5 / ASK1 Antibody - Anti-MAP3K5 / ASK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   1mg/ml   $545.00
MAP3K5 / ASK1 Antibody - Anti-MAP3K5 / ASK1 antibody IHC of human skin, sweat ducts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $425.00
MAP3K5 / ASK1 Antibody - Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.
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Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, WB
Unconjugated
200µg   1mg/ml   $445.00
MAP3K5 / ASK1 Antibody - Western blot of ASK 1 antibody
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Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
MAP3K5 / ASK1 Antibody - Immunohistochemical analysis of ASK1 (pS83) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Rabbit Polyclonal to Human MAP3K5 / ASK1
Mouse, Bovine, Rat, Human
IHC, IHC-P, WB
Unconjugated
30µl   1mg/ml   $215.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $325.00
MAP3K5 / ASK1 Antibody - MAP3K5 monoclonal antibody (M01), clone 5C4 Western Blot analysis of MAP3K5 expression in HepG2.
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Mouse Monoclonal [clone 5C4] (IgG2b,k) to Human MAP3K5 / ASK1
Human
ELISA, WB
Unconjugated
100µg   $365.00
MAP3K5 / ASK1 Antibody - IHC of p-ASK1 (S966)pAb in paraffin-embedded human breast carcinoma tissue.
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Rabbit Polyclonal to Human MAP3K5 / ASK1
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl   $345.00
MAP3K5 / ASK1 Antibody - Immunohistochemistry (IHC) analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) with DAB staining using ASK 1 Monoclonal Antibody.
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Mouse Monoclonal (IgG1) to Human MAP3K5 / ASK1
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
100µl   $345.00
MAP3K5 / ASK1 Antibody - ASK1 Antibody in Immunohistochemistry (IHC)
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Mouse Monoclonal [clone 20000] (IgG1) to Human MAP3K5 / ASK1
Human
Flo, ICC, IF, IHC, WB
Unconjugated
100µl   $425.00
MAP3K5 / ASK1 Antibody - IHC of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse monoclonal antibody with DAB staining.
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Mouse Monoclonal [clone 2E4] (IgG1) to Human MAP3K5 / ASK1
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
100µl   $365.00
Select
Mouse Monoclonal (IgG1) to Human MAP3K5 / ASK1
Human
ELISA, Flo, ICC, IHC, WB
Unconjugated
50µl   1mg/ml   $315.00; 100µl   1mg/ml   $375.00; 200µl   1mg/ml   $435.00
MAP3K5 / ASK1 Antibody - 1:100 staining human lymph node tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
MAP3K5 / ASK1 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
MAP3K5 / ASK1 Antibody - 1:100 staining human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
MAP3K5 / ASK1 Antibody - Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 µg/ml.
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Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, ICC, IF, WB
Unconjugated
100µg   $365.00
MAP3K5 / ASK1 Antibody - Immunohistochemistry of paraffin-embedded human testis tissue using MAP3K5 Antibody at dilution of 1:100
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Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IF, IHC
Unconjugated
50µg   $285.00; 100µg   $385.00
MAP3K5 / ASK1 Antibody - Western blot analysis of extracts of various cell lines.
Select
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl   $235.00; 100µl   $315.00; 200µl   $425.00
Select
Rabbit Polyclonal (IgG) to Mouse MAP3K5 / ASK1
Mouse, Human
ELISA, IHC, WB
Unconjugated
200µl   $590.00
MAP3K5 / ASK1 Antibody - Mouse Map3k5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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Rabbit Polyclonal to Mouse MAP3K5 / ASK1
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl   $325.00
MAP3K5 / ASK1 Antibody - Formalin-fixed paraffin-embedded tissue section of human reactive tonsil stained for ASK1 expression using Polyclonal Antibody to ASK1 at 1:2000. A and A1. Low and high magnification, respectively. ASK1 expression was seen in the vasculature (endothelial cells). Hematoxylin-Eosin counterstain.
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Rabbit Polyclonal to Human MAP3K5 / ASK1
Human
IHC, IHC-Fr, IHC-P, IP, WB
Unconjugated
50µl   $335.00
MAP3K5 / ASK1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ASK1 (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.
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Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
MAP3K5 / ASK1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ASK1 (Phospho-Ser966) Antibody. The picture on the right is blocked with the phospho peptide.
Select
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
Select
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µg   $435.00
Select
Rabbit Polyclonal (IgG) to Human MAP3K5 / ASK1
Human
ELISA, IHC, WB
Unconjugated
50µg   $435.00
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If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).