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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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MAP3K5 / ASK1 Antibody LS‑C271

Catalog Number Size Price
LS-C271-0.1 0.1 mg $365 
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 µg/ml.
Immunofluorescence of ASK1 in A431 cells with ASK1 antibody at 20 ug/ml.
Western blot analysis ASK1 in SW1353 whole cell lysate with ASK1 antibody at 0.5 µg/ml.
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 µg/ml.
Immunofluorescence of ASK1 in A431 cells with ASK1 antibody at 20 ug/ml.
Western blot analysis ASK1 in SW1353 whole cell lysate with ASK1 antibody at 0.5 µg/ml.
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MAP3K5 / ASK1 Antibody LS‑C271

Antibody:
Rabbit Polyclonal to Human MAP3K5 / ASK1
Application:
ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365 
LS-C271-0.1
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human MAP3K5 / ASK1
Application:
ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ASK1 antibody LS-C271 is an unconjugated rabbit polyclonal antibody to human ASK1 (MAP3K5 ). Validated for ELISA, ICC, IF and WB.
Target
Human MAP3K5 / ASK1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • ELISA
Failed Applications
  • IHC - Paraffin
Immunogen
a peptide corresponding to amino acids near the C-terminus of human ASK1. The sequence is different from that of mouse by last two amino acids. The immunogen is located within the last 50 amino acids of ASK1.
Blocking Peptide
LS-E29855 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-C271. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 ug/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 155 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP3K5 / ASK1
Q99683 NM_005923 NP_005914.1

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Requested From: United States
Date Requested: 8/19/2019
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