LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

MAP3K5 / ASK1 Antibody (phospho-Ser83) LS-C18846

ASK1 antibody LS-C18846 is an unconjugated rabbit polyclonal antibody to human ASK1 (MAP3K5). Validated for ELISA and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C18846-200
200 µg (1 mg/ml)
$445
Description
ASK1 antibody LS-C18846 is an unconjugated rabbit polyclonal antibody to human ASK1 (MAP3K5). Validated for ELISA and WB. Cited in 1 publication.
Target
Human MAP3K5 / ASK1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA (1:5000 - 1:10000)
Epitope
pSer83
Specificity
KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Usage
This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. A 155 kD band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Restrictions
For research use only.
About MAP3K5 / ASK1
Q99683 NM_005923 NP_005914.1

Popular MAP3K5 / ASK1 Products

Anti-MAP3K5 / ASK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Anti-MAP3K5 / ASK1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
MAP3K5 monoclonal antibody (M01), clone 5C4 Western Blot analysis of MAP3K5 expression in HepG2.
Species: Human
Applications: Western blot, ELISA
IHC of p-ASK1 (S966)pAb in paraffin-embedded human breast carcinoma tissue.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western blot of ASK 1 antibody
Species: Human
Applications: IHC, Western blot, ELISA

Publications (1)

1
Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase. Subramanyam M, Takahashi N, Hasegawa Y, Mohri T, Okada Y. The Journal of biological chemistry. 2010 285:6109-17. [PubMed:20048146] [PMC:PMC2825405]

Customer Reviews (0)


Images

Western blot

Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.
Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.

Enzyme-Linked Immunosorbent Assay

Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.
Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.

Western blot

Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.
Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.

Enzyme-Linked Immunosorbent Assay

Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.
Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.

Western blot

Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.
Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.

Enzyme-Linked Immunosorbent Assay

Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.
Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.

Western blot

Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.
Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.

Enzyme-Linked Immunosorbent Assay

Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.
Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.

Requested From: United States
Date Requested: 4/21/2019
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2019 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy