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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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hCG / Chorionic Gonadotropin

hCG / Human Chorionic Gonadotropin


hCG / Chorionic Gonadotropin Target Details

Target Name: hCG / Human Chorionic Gonadotropin
Target Abbreviation: hCG

Filtered By: None
Target
hcg-chorionic-gonadotropin (66)
Reactivity
Human (65)
Mouse (2)
Rat (1)
Horse (1)
Application
IHC (18)
IHC-Fr (2)
IHC-P (12)
WB (14)
ELISA (35)
ICC (8)
IHC-Fx (1)
IP (24)
RIA (27)
Host
rabbit (20)
mouse (38)
goat (1)
chicken (5)
sheep (2)
Isotype
IgG (20)
IgG1 (27)
IgG1,k (7)
IgG1,k + IgG2a,k (1)
IgY (1)
Clonality
monoclonal mc (38)
polyclonal pc (28)
Clone
3002 (2)
3015 (2)
34P2C2 (2)
BSB-38 (1)
CG04 + CG05 (2)
h 6 (1)
HCGab/52 (3)
INN-hCG-45 (1)
INN-hCG-53 (1)
P1G7F12 (2)
P3G7 (2)
P6F11G5 (2)
SC1 (2)
SC2 (2)
SPM105 (1)
Format
AP Conjugated (2)
APC (1)
APC Conjugated (2)
Azide-free (2)
Biotin Conjugated (3)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (4)
HRP Conjugated (5)
PE Conjugated (3)
Ready-to-Use (2)
Unconjugated (45)
Publications
No (66)
Showing 1-25 of 66 results

hCG / Chorionic Gonadotropin Antibody (clone CG04 + CG05) IHC-plus™ LS-B8506
Mouse Monoclonal [clone CG04 + CG05] (IgG1,k + IgG2a,k) to Human hCG / Chorionic Gonadotropin
Human
IHC IHC-P
Unconjugated
250µl  $425.00
Anti-Chorionic Gonadotropin antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8506 dilution 10 ug/ml.
Anti-Chorionic Gonadotropin antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8506 dilution 10 ug/ml.

hCG / Chorionic Gonadotropin Antibody LS-C128164
Rabbit Polyclonal to Horse hCG / Chorionic Gonadotropin
Horse
RIA WB
Unconjugated
20µl  $405.00; 100µl  $725.00

hCG / Chorionic Gonadotropin Antibody LS-C293326
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ICC IHC IP WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $295.00; 1ml 0.5mg/ml  $515.00; 10ml 0.5mg/ml  $2,040.00

hCG / Chorionic Gonadotropin Antibody (HRP) LS-C712338
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ICC IHC IP WB
HRP Conjugated
20µl  $215.00; 100µl  $285.00; 200µl  $335.00; 1ml  $665.00; 10ml  $2,640.00

hCG / Chorionic Gonadotropin Antibody (Biotin) LS-C305786
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ICC IHC IP WB
Biotin Conjugated
100µl 0.45mg/ml  $325.00

hCG / Chorionic Gonadotropin Antibody (FITC) LS-C694642
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ICC IHC IP WB
FITC Conjugated
20µl  $205.00; 100µl  $275.00; 200µl  $325.00; 1ml  $605.00; 10ml  $2,445.00

hCG / Chorionic Gonadotropin Antibody (Cy3) LS-C703314
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ICC IHC IP WB
Cy3 Conjugated
20µl  $245.00; 100µl  $355.00; 200µl  $435.00; 1ml  $1,020.00; 10ml  $4,080.00

hCG / Chorionic Gonadotropin Antibody (PE) LS-C726400
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ICC IHC IP WB
PE Conjugated
20µl  $205.00; 100µl  $275.00; 200µl  $325.00; 1ml  $605.00; 10ml  $2,445.00

hCG / Chorionic Gonadotropin Antibody (APC, Cy7) LS-C735329
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ICC IHC IP WB
APC, Cy7 Conjugated
20µl  $285.00; 100µl  $445.00; 200µl  $605.00; 1ml  $1,530.00; 10ml  $6,120.00

hCG / Chorionic Gonadotropin Antibody (APC) LS-C740965
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ICC IHC IP WB
APC Conjugated
20µl  $225.00; 100µl  $305.00; 200µl  $365.00; 1ml  $770.00

hCG / Chorionic Gonadotropin Antibody (clone SPM105) IHC-plus™ LS-B8636
Mouse Monoclonal [clone SPM105] (IgG1,k) to Human hCG / Chorionic Gonadotropin
Human
IHC IHC-P
Unconjugated
250µl  $425.00
Anti-Chorionic Gonadotropin antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8636 dilution 10 ug/ml.
Anti-Chorionic Gonadotropin antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8636 dilution 10 ug/ml.

hCG / Chorionic Gonadotropin Antibody (clone INN-hCG-53) LS-C57373
Mouse Monoclonal [clone INN-hCG-53] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA WB
Unconjugated
500µg 500µl 1mg/ml  $385.00

hCG / Chorionic Gonadotropin Antibody LS-C22144
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
ELISA
Unconjugated
1000µg 6.2mg/ml  $455.00

hCG / Chorionic Gonadotropin Antibody IHC-plus™ LS-B9353
Rabbit Polyclonal (IgG) to Human hCG / Chorionic Gonadotropin
Human
IHC IHC-Fx IHC-P
Unconjugated
250µl  $425.00
Anti-hCG / Chorionic Gonadotropin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-hCG / Chorionic Gonadotropin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

hCG / Chorionic Gonadotropin Antibody (clone SC1) LS-C355428
Mouse Monoclonal [clone SC1] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
1000µg  $650.00
Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
Western blot analysis of hCG was performed by loading the indicated amounts of protein purified hCG protein extracted from human urine, and 15ul of PageRuler Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using a anti hCG antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
Western blot analysis of hCG was performed by loading the indicated amounts of protein purified hCG protein extracted from human urine, and 15ul of PageRuler Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using a anti hCG antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

hCG / Chorionic Gonadotropin Antibody (clone SC1) LS-C355244
Mouse Monoclonal [clone SC1] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
100µg  $425.00
Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (right panel) or with hCG antibody (left panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using a DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
Western blot analysis of hCG was performed by loading the indicated amounts of purified hCG protein extracted from human urine, and 15ul of PageRuler Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using a anti hCG antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (right panel) or with hCG antibody (left panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using a DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
Western blot analysis of hCG was performed by loading the indicated amounts of purified hCG protein extracted from human urine, and 15ul of PageRuler Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using a anti hCG antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

hCG / Chorionic Gonadotropin Antibody (clone 3002) LS-C355256
Mouse Monoclonal [clone 3002] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
100µg  $405.00
Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extracellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (right panel) or with hCG antibody (left panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using a DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
Immunoprecipitation of hCG proteins was performed by spiking 5 ugs of purified hCG protein extracted from human urine into 1ml of 1:2 diluted human urine sample. Antigen-antibody complexes were formed by incubating with 5ug of hCG antibody overnight on end over end tube rotator at 4C. The immune complexes were captured by using 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using hCG monoclonal antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extracellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (right panel) or with hCG antibody (left panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using a DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
Immunoprecipitation of hCG proteins was performed by spiking 5 ugs of purified hCG protein extracted from human urine into 1ml of 1:2 diluted human urine sample. Antigen-antibody complexes were formed by incubating with 5ug of hCG antibody overnight on end over end tube rotator at 4C. The immune complexes were captured by using 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using hCG monoclonal antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

hCG / Chorionic Gonadotropin Antibody (clone 3002) LS-C355424
Mouse Monoclonal [clone 3002] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
1000µg  $650.00
Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
Immunoprecipitation of hCG proteins was performed by spiking 5 ugs of purified hCG protein extracted from human urine into 1ml of 1:2 diluted human urine sample. Antigen-antibody complexes were formed by incubating with 5ug of hCG antibody overnight on end over end tube rotator at 4C. The immune complexes were captured by using 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using hCG monoclonal antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
Immunoprecipitation of hCG proteins was performed by spiking 5 ugs of purified hCG protein extracted from human urine into 1ml of 1:2 diluted human urine sample. Antigen-antibody complexes were formed by incubating with 5ug of hCG antibody overnight on end over end tube rotator at 4C. The immune complexes were captured by using 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using hCG monoclonal antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

hCG / Chorionic Gonadotropin Antibody (clone BSB-38, Ready-to-Use) LS-C348658
Mouse Monoclonal [clone BSB-38] (IgG1,k) to Human hCG / Chorionic Gonadotropin
Human
IHC IHC-Fr IHC-P
Unconjugated, Ready-to-Use
3000µl  $195.00; 7000µl  $235.00; 15000µl  $275.00
IHC of hCG on FFPE Placenta tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC of hCG on FFPE Placenta tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.

hCG / Chorionic Gonadotropin Antibody LS-C312062
Rabbit Polyclonal to Human hCG / Chorionic Gonadotropin
Human
IHC IHC-Fr IHC-P
Unconjugated
100µl  $225.00; 500µl  $275.00; 1000µl  $345.00
IHC of hCG on FFPE Placenta tissue.
IHC of hCG on FFPE Placenta tissue.

hCG / Chorionic Gonadotropin Antibody (clone P6F11G5) LS-C355294
Mouse Monoclonal [clone P6F11G5] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
100µg  $405.00

hCG / Chorionic Gonadotropin Antibody (clone P6F11G5) LS-C355432
Mouse Monoclonal [clone P6F11G5] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
1000µg  $650.00

hCG / Chorionic Gonadotropin Antibody (clone P3G7) LS-C355435
Mouse Monoclonal [clone P3G7] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
1000µg  $650.00

hCG / Chorionic Gonadotropin Antibody (clone P3G7) LS-C355245
Mouse Monoclonal [clone P3G7] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
100µg  $405.00

hCG / Chorionic Gonadotropin Antibody (clone P1G7F12) LS-C355260
Mouse Monoclonal [clone P1G7F12] (IgG1) to Human hCG / Chorionic Gonadotropin
Human
ELISA IP RIA
Unconjugated
100µg  $405.00
Direct ELISA analysis of hCG proteins was performed by coating wells of a plate with a purified hCG protein extracted from human urine at a concentration of 10 µg/mL overnight at 4C. The plate was washed 3 times with ELISA Wash Buffer and blocked with 300 ul of StartingBlock (PBS) Blocking Buffer for at least 1 hour at room temperature. After washing 100ul of hCG monoclonal antibody was added to wells in duplicate at 10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 9.5, 0.078, 0.039, 0.019 and 0 µg/mL concentrations, and the samples were incubated for 2 hours at room temperature. The plate was washed, and then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, and washed again with ELISA Wash Buffer. The plate was developed by incubating 100ul per well of 1-Step Ultra TMB substrate per well for 10 minutes at room temperature in the dark. The reaction was stopped with 100ul per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
Direct ELISA analysis of hCG proteins was performed by coating wells of a plate with a purified hCG protein extracted from human urine at a concentration of 10 µg/mL overnight at 4C. The plate was washed 3 times with ELISA Wash Buffer and blocked with 300 ul of StartingBlock (PBS) Blocking Buffer for at least 1 hour at room temperature. After washing 100ul of hCG monoclonal antibody was added to wells in duplicate at 10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 9.5, 0.078, 0.039, 0.019 and 0 µg/mL concentrations, and the samples were incubated for 2 hours at room temperature. The plate was washed, and then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, and washed again with ELISA Wash Buffer. The plate was developed by incubating 100ul per well of 1-Step Ultra TMB substrate per well for 10 minutes at room temperature in the dark. The reaction was stopped with 100ul per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
Showing 1-25 of 66 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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