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Catalog Number Size Price
LS-C355441-1 1 mg $801 
hCG / Chorionic Gonadotropin Antibody - Direct ELISA analysis of hCG proteins was performed by coating wells of a plate with a purified hCG protein extracted from human urine at a concentration of 10 µg/mL overnight at 4C. The plate was washed 3 times with ELISA Wash Buffer and blocked with 300 ul of StartingBlock (PBS) Blocking Buffer for at least 1 hour at room temperature. After washing 100ul of hCG monoclonal antibody was added to wells in duplicate at 10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 9.5, 0.078, 0.039, 0.019 and 0 µg/mL concentrations, and the samples were incubated for 2 hours at room temperature. The plate was washed, and then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, and washed again with ELISA Wash Buffer. The plate was developed by incubating 100ul per well of 1-Step Ultra TMB substrate per well for 10 minutes at room temperature in the dark. The reaction was stopped with 100ul per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.

Monoclonal Mouse anti‑Human hCG / Chorionic Gonadotropin Antibody (clone P1G7F12) LS‑C355441

Monoclonal Mouse anti‑Human hCG / Chorionic Gonadotropin Antibody (clone P1G7F12) LS‑C355441

Antibody:
hCG / Chorionic Gonadotropin Mouse anti-Human Monoclonal (P1G7F12) Antibody
Application:
IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$801
LS-C355441-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
hCG / Chorionic Gonadotropin Mouse anti-Human Monoclonal (P1G7F12) Antibody
Application:
IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Chorionic Gonadotropin antibody LS-C355441 is an unconjugated mouse monoclonal antibody to human Chorionic Gonadotropin (hCG). Validated for ELISA, IP and RIA.
Target
Human hCG / Chorionic Gonadotropin
Synonyms
hCG
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
P1G7F12
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Chorionic Gonadotropin, beta-subunit
Specificity
The antibody targets Chorionic Gonadotropin in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Chorionic Gonadotropin which has a predicted molecular weight of approximately 13 kD.
Applications
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024