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Antibodies > hCG / Chorionic Gonadotropin > LS-C355429
 
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Catalog Number Size Price
LS-C355429-1 1 mg $801 
hCG / Chorionic Gonadotropin Antibody - Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
hCG / Chorionic Gonadotropin Antibody - Western blot analysis of hCG was performed by loading the indicated amounts of protein purified hCG protein extracted from human urine, and 15ul of PageRuler Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~22kD using an anti hCG antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
hCG / Chorionic Gonadotropin Antibody - Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
hCG / Chorionic Gonadotropin Antibody - Western blot analysis of hCG was performed by loading the indicated amounts of protein purified hCG protein extracted from human urine, and 15ul of PageRuler Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~22kD using an anti hCG antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
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Monoclonal Mouse anti‑Human hCG / Chorionic Gonadotropin Antibody (clone 3015, IHC, WB) LS‑C355429

Monoclonal Mouse anti‑Human hCG / Chorionic Gonadotropin Antibody (clone 3015, IHC, WB) LS‑C355429

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Antibody:
hCG / Chorionic Gonadotropin Mouse anti-Human Monoclonal (3015) Antibody
Application:
IHC, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$801
LS-C355429-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
hCG / Chorionic Gonadotropin Mouse anti-Human Monoclonal (3015) Antibody
Application:
IHC, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Chorionic Gonadotropin antibody LS-C355429 is an unconjugated mouse monoclonal antibody to human Chorionic Gonadotropin (hCG). Validated for ELISA, IHC, IP, RIA and WB.
Target
Human hCG / Chorionic Gonadotropin
Synonyms
hCG
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
3015
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Human Chorionic Gonadotropin
Specificity
The antibody targets Chorionic Gonadotropin in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Chorionic Gonadotropin which has a predicted molecular weight of approximately 13 kD.
Applications
  • IHC
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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hCG / Chorionic Gonadotropin ELISA Kit
Human hCG / Chorionic Gonadotropin (Sandwich ELISA) ELISA Kit - LS-F10005
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Range: 8-240 mIU/ml
See all hCG / Chorionic Gonadotropin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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