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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSTA / Cystatin A

cystatin A (stefin A)

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Gene Name: cystatin A (stefin A)
Synonyms: CSTA, AREI, Cystatin A (stefin A), Cystatin A, Cystatin-A, STFA, Stefin-A, STF1, Cystatin AS, Cystatin-AS
Target Sequences: NM_005213 NP_005204.1 P01040

Filtered by: None
Target
CSTA / Cystatin A (71)
Reactivity
Human (55)
Mouse (10)
Rat (9)
Bat (1)
Bovine (1)
Dog (1)
Horse (1)
Pig (1)
Sheep (1)
Application
IHC (24)
IHC-P (13)
WB (63)
Flo (2)
ELISA (23)
ICC (13)
IF (7)
IP (9)
Peptide-ELISA (1)
Host
rabbit (55)
mouse (16)
Isotype
IgG (31)
IgG1 (2)
IgG1,k (2)
IgG2b,k (1)
Clonality
monoclonal mc (16)
polyclonal pc (55)
Clone
1A11 (1)
AT1B12 (1)
PAT1B12AT (1)
Format
AP Conjugated (1)
APC (4)
APC Conjugated (5)
Azide-free (1)
Biotin Conjugated (7)
Cy3 Conjugated (4)
Cy7 Conjugated (4)
FITC Conjugated (7)
HRP Conjugated (6)
PE Conjugated (5)
Unconjugated (32)
Epitope
aa1-98 (18)
aa1-103 (8)
aa1-97 (8)
aa69-98 (8)
20-100 aa, Internal (1)
Gly84 (1)
Internal (1)
aa49-98 (1)
aa77-90 (1)
aa8-98 (1)
Publications
No (71)
Showing 1-25 of 71 results

IHC-plus™ CSTA / Cystatin A Antibody (aa49-98) LS-B8392
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-Cystatin A / CSTA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Immunofluorescence analysis of A549 cells, using Stefin A Antibody. The picture on the right is blocked with the synthesized peptide.

CSTA / Cystatin A Antibody (Internal) LS-C413052
Rabbit Polyclonal to Human CSTA / Cystatin A
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of Cystatin A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Cystatin A staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CSTA / Cystatin A Antibody (aa1-97, FITC) LS-C302524
Rabbit Polyclonal to Mouse CSTA / Cystatin A
Mouse
WB
FITC Conjugated
100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $575.00
Western blot of recombinant CSTA / Cystatin A.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CSTA / Cystatin A Antibody LS-C806703
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

CSTA / Cystatin A Antibody (clone PAT1B12AT) LS-C760740
Mouse Monoclonal [clone PAT1B12AT] (IgG1,k) to Human CSTA / Cystatin A
Human
ELISA, Flo, ICC, WB
Unconjugated
5µg  $195.00; 20µg  $255.00; 100µg  $475.00

CSTA / Cystatin A Antibody (20-100 aa, Internal) LS-C383048
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Cystatin A antibody

CSTA / Cystatin A Antibody (clone AT1B12) LS-C755437
Mouse Monoclonal [clone AT1B12] (IgG1,k) to Human CSTA / Cystatin A
Human
ELISA, Flo, ICC, WB
Unconjugated
50µl 1mg/ml  $275.00; 100µl 1mg/ml  $345.00
ICC/IF analysis of CSTA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CSTA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A431 cell lysate Lane 2.: THP1 cell lysate

CSTA / Cystatin A Antibody (aa1-98, Biotin) LS-C298829
Rabbit Polyclonal to Human CSTA / Cystatin A
Human
WB
Biotin Conjugated
100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $295.00; 1ml 0.5mg/ml  $515.00
Western blot of recombinant CSTA / Cystatin A.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot; Sample: Recombinant CSTA, Human.

CSTA / Cystatin A Antibody (Gly84) LS-C176559
Rabbit Polyclonal to Human CSTA / Cystatin A
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of Cystatin A (G84) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of Cystatin A (G84) pAb in extracts from HT-29 cells.

CSTA / Cystatin A Antibody LS-C334215
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of THP-1 cells.

CSTA / Cystatin A Antibody LS-C664946
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
ELISA, IF, WB
Unconjugated
100µg  $335.00

CSTA / Cystatin A Antibody (aa69-98) LS-C167108
Rabbit Polyclonal to Human CSTA / Cystatin A
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
CSTA Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skin tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of CSTA (arrow) using rabbit polyclonal CSTA Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CSTA gene.

CSTA / Cystatin A Antibody (aa69-98) LS-C329434
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

CSTA / Cystatin A Antibody (aa69-98, Biotin) LS-C208682
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
ELISA, IHC, WB
Biotin Conjugated
200µl  $740.00

CSTA / Cystatin A Antibody (aa69-98, AP) LS-C208680
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
ELISA, IHC, WB
AP Conjugated
200µl  $740.00

CSTA / Cystatin A Antibody (aa69-98, APC) LS-C208681
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
ELISA, IHC, WB
APC Conjugated
200µl  $740.00

CSTA / Cystatin A Antibody (aa69-98, Azide-free, HRP) LS-C208684
Rabbit Polyclonal (IgG) to Human CSTA / Cystatin A
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

CSTA / Cystatin A Antibody (aa77-90) LS-C313223
Rabbit Polyclonal to Human CSTA / Cystatin A
Human
IHC, IHC-P, WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
CSTA / Cystatin A antibody. IHC(P): Human Tonsil Tissue.
WB of CSTA / Cystatin A antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human CSTA, 45.0KD. (M1-F98+unrelated protein,311aa). . Lane 1: Recombinant Human CSTA Protein 10ng. Lane 2: Recombinant Human CSTA Protein 5ng. Lane 3: Recombinant Human CSTA Protein 2.5ng. Lane 4: Recombinant Human CSTA Protein 1.25ng..

CSTA / Cystatin A Antibody (aa8-98) LS-C407777
Rabbit Polyclonal to Human CSTA / Cystatin A
Dog, Sheep, Bovine, Rat, Pig, Horse, Bat, Human
IHC, IHC-P, WB
Unconjugated
100µg  $325.00
Cystatin A antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
Cystatin A antibody Western blot. All lanes: Anti Cystatin A at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 54 kD. Observed band size: 54 kD.

CSTA / Cystatin A Antibody LS-C22587
Mouse Monoclonal (IgG1) to Human CSTA / Cystatin A
Human
IHC, IHC-P, WB
Unconjugated
200µg  $710.00

CSTA / Cystatin A Antibody (aa1-98) LS-C314178
Mouse Monoclonal (IgG) to Human CSTA / Cystatin A
Human
ICC, IP, WB
Unconjugated
20µl 1mg/ml  $205.00; 100µl 1mg/ml  $255.00; 200µl 1mg/ml  $345.00; 1ml 1mg/ml  $495.00
Western blot of recombinant CSTA / Cystatin A.

CSTA / Cystatin A Antibody LS-C485766
Rabbit Polyclonal to Human CSTA / Cystatin A
Human
ELISA, IHC, IHC-P
Unconjugated
100µl  $255.00; 200µl  $305.00; 400µl  $355.00

CSTA / Cystatin A Antibody LS-C485764
Rabbit Polyclonal to Human CSTA / Cystatin A
Human
ELISA, IHC, IHC-P
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

CSTA / Cystatin A Antibody LS-C485767
Rabbit Polyclonal to Human CSTA / Cystatin A
Human
ELISA, IHC, IHC-P
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

CSTA / Cystatin A Antibody LS-C48466
Mouse Monoclonal (IgG1) to Human CSTA / Cystatin A
Human
IHC, WB
Unconjugated
100µg 100µl  $455.00
Showing 1-25 of 71 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).