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Catalog Number Size Price
LS-C313223-10 10 µg $318 
LS-C313223-100 100 µg (0.5 mg/ml) $470 
CSTA / Cystatin A Antibody - CSTA / Cystatin A antibody. IHC(P): Human Tonsil Tissue.
CSTA / Cystatin A Antibody - Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
CSTA / Cystatin A Antibody - WB of CSTA / Cystatin A antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human CSTA, 45.0KD. (M1-F98+unrelated protein,311aa). . Lane 1: Recombinant Human CSTA Protein 10ng. Lane 2: Recombinant Human CSTA Protein 5ng. Lane 3: Recombinant Human CSTA Protein 2.5ng. Lane 4: Recombinant Human CSTA Protein 1.25ng..
CSTA / Cystatin A Antibody - CSTA / Cystatin A antibody. IHC(P): Human Tonsil Tissue.
CSTA / Cystatin A Antibody - Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
CSTA / Cystatin A Antibody - WB of CSTA / Cystatin A antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human CSTA, 45.0KD. (M1-F98+unrelated protein,311aa). . Lane 1: Recombinant Human CSTA Protein 10ng. Lane 2: Recombinant Human CSTA Protein 5ng. Lane 3: Recombinant Human CSTA Protein 2.5ng. Lane 4: Recombinant Human CSTA Protein 1.25ng..
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Polyclonal Rabbit anti‑Human CSTA / Cystatin A Antibody (aa77‑90, IHC, WB) LS‑C313223

Polyclonal Rabbit anti‑Human CSTA / Cystatin A Antibody (aa77‑90, IHC, WB) LS‑C313223

Note: This antibody replaces LS-C388325
Antibody:
CSTA / Cystatin A Rabbit anti-Human Polyclonal (aa77-90) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313223-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CSTA / Cystatin A Rabbit anti-Human Polyclonal (aa77-90) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cystatin A antibody LS-C313223 is an unconjugated rabbit polyclonal antibody to human Cystatin A (CSTA) (aa77-90). Validated for IHC and WB.
Target
Human CSTA / Cystatin A
Synonyms
CSTA | AREI | Cystatin A (stefin A) | Cystatin A | Cystatin-A | STFA | Stefin-A | STF1 | Cystatin AS | Cystatin-AS
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Cystatin A(77-90 aa NEDLVLTGYQVDKN).
Epitope
aa77-90
Specificity
Expressed in the skin throughout the epidermis. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CSTA / Cystatin A
P01040 NM_005213 NP_005204.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/24/2024