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Catalog Number Size Price
LS-C407777-10 10 µg $318 
LS-C407777-100 100 µg $470 
CSTA / Cystatin A Antibody - Cystatin A antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CSTA / Cystatin A Antibody - Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
CSTA / Cystatin A Antibody - Cystatin A antibody Western blot. All lanes: Anti Cystatin A at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 54 kD. Observed band size: 54 kD.
CSTA / Cystatin A Antibody - Cystatin A antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CSTA / Cystatin A Antibody - Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
CSTA / Cystatin A Antibody - Cystatin A antibody Western blot. All lanes: Anti Cystatin A at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 54 kD. Observed band size: 54 kD.
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Polyclonal Rabbit anti‑Human CSTA / Cystatin A Antibody (aa8‑98, IHC, WB) LS‑C407777

Polyclonal Rabbit anti‑Human CSTA / Cystatin A Antibody (aa8‑98, IHC, WB) LS‑C407777

Antibody:
CSTA / Cystatin A Rabbit anti-Human Polyclonal (aa8-98) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407777-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CSTA / Cystatin A Rabbit anti-Human Polyclonal (aa8-98) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
Cystatin A antibody LS-C407777 is an unconjugated rabbit polyclonal antibody to Cystatin A (CSTA) (aa8-98) from human. It is reactive with human, rat, bat and other species. Validated for IHC and WB.
Target
Human CSTA / Cystatin A
Synonyms
CSTA | AREI | Cystatin A (stefin A) | Cystatin A | Cystatin-A | STFA | Stefin-A | STF1 | Cystatin AS | Cystatin-AS
Host
Rabbit
Reactivity
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Cystatin A recombinant protein (Position: E8-F98). Human Cystatin A shares 61.5% and 58.2% amino acid (aa) sequence identity with mouse and rat Cystatin A, respectively.
Epitope
aa8-98
Specificity
Expressed in the skin throughout the epidermis. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CSTA / Cystatin A
P01040 NM_005213 NP_005204.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024