Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C407777-10 10 µg $318 
LS-C407777-100 100 µg $470 
CSTA / Cystatin A Antibody - Cystatin A antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CSTA / Cystatin A Antibody - Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
CSTA / Cystatin A Antibody - Cystatin A antibody Western blot. All lanes: Anti Cystatin A at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 54 kD. Observed band size: 54 kD.
CSTA / Cystatin A Antibody - Cystatin A antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CSTA / Cystatin A Antibody - Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
CSTA / Cystatin A Antibody - Cystatin A antibody Western blot. All lanes: Anti Cystatin A at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 54 kD. Observed band size: 54 kD.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human CSTA / Cystatin A Antibody (aa8‑98, IHC, WB) LS‑C407777

Polyclonal Rabbit anti‑Human CSTA / Cystatin A Antibody (aa8‑98, IHC, WB) LS‑C407777

Antibody:
CSTA / Cystatin A Rabbit anti-Human Polyclonal (aa8-98) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407777-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CSTA / Cystatin A Rabbit anti-Human Polyclonal (aa8-98) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
Cystatin A antibody LS-C407777 is an unconjugated rabbit polyclonal antibody to Cystatin A (CSTA) (aa8-98) from human. It is reactive with human, rat, bat and other species. Validated for IHC and WB.
Target
Human CSTA / Cystatin A
Synonyms
CSTA | AREI | Cystatin A (stefin A) | Cystatin A | Cystatin-A | STFA | Stefin-A | STF1 | Cystatin AS | Cystatin-AS
Host
Rabbit
Reactivity
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Cystatin A recombinant protein (Position: E8-F98). Human Cystatin A shares 61.5% and 58.2% amino acid (aa) sequence identity with mouse and rat Cystatin A, respectively.
Epitope
aa8-98
Specificity
Expressed in the skin throughout the epidermis. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CSTA / Cystatin A
P01040 NM_005213 NP_005204.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024