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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CEACAM5 / CD66e

carcinoembryonic antigen-related cell adhesion molecule 5

Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.

Gene Name: carcinoembryonic antigen-related cell adhesion molecule 5
Family/Subfamily: CEA , not assigned-CEA
Synonyms: CEACAM5, CD66e, CD66e antigen, CEA, Carcinoembryonic antigen, Meconium antigen 100
Target Sequences: NM_004363 NP_004354.3 P06731

Filtered by: None
Target
CEACAM5 / CD66e (82)
Reactivity
Human (81)
Mouse (6)
Rat (4)
Application
IHC (46)
IHC-Fr (9)
IHC-P (24)
WB (44)
Flo (24)
ELISA (34)
ICC (4)
IF (13)
IP (8)
RIA (4)
Host
rabbit (34)
mouse (48)
Isotype
IgG (15)
IgG1 (25)
IgG1,k (3)
IgG2a (5)
IgG2a,k (3)
IgG2b (6)
Clonality
monoclonal mc (49)
polyclonal pc (33)
Clone
10E1 (1)
1105 (2)
1106 (2)
1A2 (1)
1C7 (1)
26/3/13 (1)
2C3 (1)
3G12 (1)
ABT-CEA (1)
C365D3(NCRC23) (2)
C66/1009 (3)
C66/1030 (3)
CB30 (7)
CEA31 (1)
CLB-139 (1)
OTI1C2 (2)
OTI1D4 (2)
OTI2B12 (2)
OTI3D8 (2)
OTI3E10 (2)
OTI5F2 (2)
OTI9F9 (2)
Format
APC Conjugated (1)
Azide-free (3)
Biotin Conjugated (2)
Carrier-free (7)
Concentrated (3)
FITC Conjugated (3)
HRP Conjugated (1)
PE Conjugated (2)
Ready-to-Use (2)
RPE Conjugated (1)
Unconjugated (72)
Epitope
aa35-680 (14)
N-Terminus (4)
471-520 aa, Internal (1)
Internal (1)
aa395-702 (1)
Publications
No (82)
Showing 1-25 of 82 results

IHC-plus™ CEACAM5 / CD66e Antibody (clone CB30) LS-B7292
Mouse Monoclonal [clone CB30] (IgG1) to Human CEACAM5 / CD66e
Human
Flo, IHC, IHC-Fr, IHC-P, IP
Unconjugated
25µg 1mg/ml  $425.00
Anti-CEA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

CEACAM5 / CD66e Antibody (clone 1105) LS-C355409
Mouse Monoclonal [clone 1105] (IgG1) to Human CEACAM5 / CD66e
Human
ELISA, Flo, ICC, IHC, IHC-P, IP, RIA, WB
Unconjugated
1000µg 2.63mg/ml  $680.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

CEACAM5 / CD66e Antibody (clone CB30, Biotin) LS-C134536
Mouse Monoclonal [clone CB30] (IgG1) to Human CEACAM5 / CD66e
Human
ELISA
Biotin Conjugated
100µg 0.5mg/ml  $345.00

CEACAM5 / CD66e Antibody (FITC) LS-C485364
Rabbit Polyclonal to Human CEACAM5 / CD66e
Human
Flo
FITC Conjugated
25tst 0.1mg/ml  $245.00; 50tst 0.1mg/ml  $295.00; 100tst 0.1mg/ml  $325.00

CEACAM5 / CD66e Antibody (clone C365D3(NCRC23), FITC) LS-C43867
Mouse Monoclonal [clone C365D3(NCRC23)] (IgG1) to Human CEACAM5 / CD66e
Human
Flo
FITC Conjugated
0.1mg  $355.00

CEACAM5 / CD66e Antibody (clone CB30) LS-C46143
Mouse Monoclonal [clone CB30] (IgG1) to Human CEACAM5 / CD66e
Human
Flo, IHC, IHC-Fr, IHC-P, IP
Unconjugated
0.1mg  $255.00
Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30).  Primary antibody dilution: 10 µg/ml.
Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30).  Primary antibody dilution: 10 µg/ml.

CEACAM5 / CD66e Antibody (clone 1C7) LS-C355356
Mouse Monoclonal [clone 1C7] (IgG1) to Human CEACAM5 / CD66e
Human
IHC, WB
Unconjugated
100µl  $425.00
IHC using CEA / CEACAM-5 Antibody (1C7)
WB using CEA / CEACAM-5 Antibody (1C7)

CEACAM5 / CD66e Antibody (471-520 aa, Internal) LS-C386940
Rabbit Polyclonal (IgG) to Human CEACAM5 / CD66e
Human
ELISA, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of CD66e antibody

IHC-plus™ CEACAM5 / CD66e Antibody (clone 26/3/13) LS-B12930
Mouse Monoclonal [clone 26/3/13] (IgG1) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IHC, IHC-Fr, IHC-P, WB
Unconjugated
50µg 2mg/ml  $485.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

CEACAM5 / CD66e Antibody (clone C365D3(NCRC23)) LS-C11572
Mouse Monoclonal [clone C365D3(NCRC23)] (IgG1) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IHC, IHC-Fr, IHC-P
Unconjugated
200µg 1mg/ml  $395.00

CEACAM5 / CD66e Antibody (aa35-680, clone OTI3D8) LS-C338757
Mouse Monoclonal [clone OTI3D8] (IgG2a) to Human CEACAM5 / CD66e
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human pancreas tissue using anti-CEACAM5 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-CEACAM5 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

CEACAM5 / CD66e Antibody LS-C313470
Rabbit Polyclonal to Human CEACAM5 / CD66e
Human
ELISA, IHC, IHC-P, WB
Unconjugated
100µg 200µl 0.5mg/ml  $355.00
WB of CEACAM5 / CD66e antibody. Lane 1: Recombinant Human CEA Protein 5ng. Lane 2: Recombinant Human CEA Protein 2.5ng. Lane 3: Recombinant Human CEA Protein 1.25ng. Lane 4: SW620 Cell Lysate.
Western blot analysis of CEA using anti-CEA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates, Lane 9: mouse SP20 whole cell lysates, Lane 10: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.

CEACAM5 / CD66e Antibody LS-C55975
Rabbit Polyclonal to Human CEACAM5 / CD66e
Human
IHC, IHC-Fr, WB
Unconjugated
50µl  $455.00

CEACAM5 / CD66e Antibody LS-C88998
Mouse Monoclonal (IgG1) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100µg  $575.00

CEACAM5 / CD66e Antibody (clone CB30, Azide-free) LS-C134538
Mouse Monoclonal [clone CB30] (IgG1) to Human CEACAM5 / CD66e
Human
ELISA
Unconjugated, Azide-free
100µg 1mg/ml  $315.00

CEACAM5 / CD66e Antibody LS-C485359
Rabbit Monoclonal to Human CEACAM5 / CD66e
Human
Flo
Unconjugated
50µl  $275.00; 100µl  $355.00

CEACAM5 / CD66e Antibody (clone 1105) LS-C355241
Mouse Monoclonal [clone 1105] (IgG1) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IP, RIA, WB
Unconjugated
100µg  $425.00
Immunofluorescent analysis of Carcinoembryonic Antigen (CEA, green) in BxPC-3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 0.3% BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a CEA monoclonal antibody at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a goat anti-mouse IgG Superclonal secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst nuclear stain. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Western blot analysis of Carcinoembryonic Antigen (CEA) was performed by loading 20ug of BxPC-3, HeLa, and HepG2 whole cell lysates per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. CEA (with varying degrees of glycosylation) was detected at ~77-180kD using a CEA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.

CEACAM5 / CD66e Antibody (clone 1106) LS-C355242
Mouse Monoclonal [clone 1106] (IgG1) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IP, RIA, WB
Unconjugated
100µg  $425.00
Immunofluorescent analysis of Carcinoembryonic Antigen (CEA, green) in BxPC-3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 0.3% BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a CEA monoclonal antibody at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a goat anti-mouse IgG Superclonal secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst nuclear stain. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Western blot analysis of Carcinoembryonic Antigen (CEA) was performed by loading 20ug of BxPC-3, HeLa, and HepG2 whole cell lysates per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% Milk in TBST for 1 hour at room temperature. CEA (with varying degrees of glycosylation) was detected at ~77-180kD using a CEA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a goat anti-mouse IgG HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.

CEACAM5 / CD66e Antibody (clone C66/1009) LS-C388241
Mouse Monoclonal [clone C66/1009] (IgG2a,k) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IHC, IHC-P
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
CEA antibody C66/1009 IHC of human colon carcinoma.  This image was taken for the unmodified form of this product. Other forms have not been tested.

CEACAM5 / CD66e Antibody (clone C66/1009, Ready-to-Use) LS-C389816
Mouse Monoclonal [clone C66/1009] (IgG2a,k) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IHC, IHC-P
Unconjugated, Ready-to-Use
7ml  $465.00
CEA antibody C66/1009 IHC of human colon carcinoma.  This image was taken for the unmodified form of this product. Other forms have not been tested.

CEACAM5 / CD66e Antibody (clone C66/1009, Azide-free) LS-C389818
Mouse Monoclonal [clone C66/1009] (IgG2a,k) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IHC, IHC-P
Unconjugated, Azide-free
100µg 1mg/ml  $465.00
CEA antibody C66/1009 IHC of human colon carcinoma.  This image was taken for the unmodified form of this product. Other forms have not been tested.

CEACAM5 / CD66e Antibody (clone C66/1030) LS-C388242
Mouse Monoclonal [clone C66/1030] (IgG1,k) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IHC, IHC-P
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
IHC CEA antibody C66/1030 colon carcinoma.  This image was taken for the unmodified form of this product. Other forms have not been tested.

CEACAM5 / CD66e Antibody (clone C66/1030, Ready-to-Use) LS-C389820
Mouse Monoclonal [clone C66/1030] (IgG1,k) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IHC, IHC-P
Unconjugated, Ready-to-Use
7ml  $465.00
IHC CEA antibody C66/1030 colon carcinoma.  This image was taken for the unmodified form of this product. Other forms have not been tested.

CEACAM5 / CD66e Antibody (clone C66/1030, Azide-free) LS-C389813
Mouse Monoclonal [clone C66/1030] (IgG1,k) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IHC, IHC-P
Unconjugated, Azide-free
100µg 1mg/ml  $465.00
IHC CEA antibody C66/1030 colon carcinoma.  This image was taken for the unmodified form of this product. Other forms have not been tested.

CEACAM5 / CD66e Antibody (clone 1106) LS-C355407
Mouse Monoclonal [clone 1106] (IgG1) to Human CEACAM5 / CD66e
Human
ELISA, Flo, IF, IP, RIA, WB
Unconjugated
1000µg  $680.00
Immunofluorescent analysis of Carcinoembryonic Antigen (CEA, green) in BxPC-3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 0.3% BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a CEA monoclonal antibody at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a goat anti-mouse IgG Superclonal secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst nuclear stain. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Western blot analysis of Carcinoembryonic Antigen (CEA) was performed by loading 20ug of BxPC-3, HeLa, and HepG2 whole cell lysates per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. CEA (with varying degrees of glycosylation) was detected at ~77-180kD using a CEA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a goat anti-mouse IgG HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.
Showing 1-25 of 82 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).