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Antibodies > CEACAM5 / CD66e > LS-C355407
 
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Catalog Number Size Price
LS-C355407-1 1 mg $857 
CEACAM5 / CD66e Antibody - Immunofluorescent analysis of Carcinoembryonic Antigen (CEA, green) in BxPC-3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 0.3% BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a CEA monoclonal antibody at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a goat anti-mouse IgG Superclonal secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst nuclear stain. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
CEACAM5 / CD66e Antibody - Western blot analysis of Carcinoembryonic Antigen (CEA) was performed by loading 20ug of BxPC-3, HeLa, and HepG2 whole cell lysates per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. CEA (with varying degrees of glycosylation) was detected at ~77-180kD using a CEA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a goat anti-mouse IgG HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.
CEACAM5 / CD66e Antibody - Flow cytometry analysis of Carcinoembryonic Antigen (CEA) on BxPC-3 cells. Cells were harvested with 0.25% Trypsin-EDTA and stained with a CEA monoclonal antibody at a dilution of 10 µg/mL (pink histogram), or with a mouse isotype control (black histogram) at a dilution of 10 µg/mL in PBS + 5% FCS. After incubation of the primary antibody for 1 hour on ice, the cells were stained with a goat anti-mouse IgG secondary antibody, DyLight 488 conjugate at a dilution of 1:40 for 1 hour on ice. A representative 10,000 cells were acquired for each sample.
CEACAM5 / CD66e Antibody - Immunofluorescent analysis of Carcinoembryonic Antigen (CEA, green) in BxPC-3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 0.3% BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a CEA monoclonal antibody at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a goat anti-mouse IgG Superclonal secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst nuclear stain. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
CEACAM5 / CD66e Antibody - Western blot analysis of Carcinoembryonic Antigen (CEA) was performed by loading 20ug of BxPC-3, HeLa, and HepG2 whole cell lysates per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. CEA (with varying degrees of glycosylation) was detected at ~77-180kD using a CEA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a goat anti-mouse IgG HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.
CEACAM5 / CD66e Antibody - Flow cytometry analysis of Carcinoembryonic Antigen (CEA) on BxPC-3 cells. Cells were harvested with 0.25% Trypsin-EDTA and stained with a CEA monoclonal antibody at a dilution of 10 µg/mL (pink histogram), or with a mouse isotype control (black histogram) at a dilution of 10 µg/mL in PBS + 5% FCS. After incubation of the primary antibody for 1 hour on ice, the cells were stained with a goat anti-mouse IgG secondary antibody, DyLight 488 conjugate at a dilution of 1:40 for 1 hour on ice. A representative 10,000 cells were acquired for each sample.
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Monoclonal Mouse anti‑Human CEACAM5 / CD66e Antibody (clone 1106, IF, WB) LS‑C355407

Monoclonal Mouse anti‑Human CEACAM5 / CD66e Antibody (clone 1106, IF, WB) LS‑C355407

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Antibody:
CEACAM5 / CD66e Mouse anti-Human Monoclonal (1106) Antibody
Application:
IF, WB, IP, Flo, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355407-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CEACAM5 / CD66e Mouse anti-Human Monoclonal (1106) Antibody
Application:
IF, WB, IP, Flo, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD66e antibody LS-C355407 is an unconjugated mouse monoclonal antibody to human CD66e (CEACAM5). Validated for ELISA, Flow, IF, IP, RIA and WB.
Target
Human CEACAM5 / CD66e
Synonyms
CEACAM5 | CD66e | CD66e antigen | CEA | Carcinoembryonic antigen | Meconium antigen 100
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1106
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Purified CEA from pooled tumors.
Specificity
The antibody targets Carcinoembryonic Antigen in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Carcinoembryonic Antigen which has a predicted molecular weight of approximately 77 kD.
Applications
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA
  • Radioimmunoassay
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CEACAM5 / CD66e
P06731 NM_004363 NP_004354.3

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CEACAM5 / CD66e Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CEACAM5 / CD66e Antibody - Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30). Primary antibody dilution: 10 µg/ml.
CEACAM5 / CD66e Antibody (clone CB30) LS‑C46143
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Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
CEACAM5 / CD66e Antibody - Western blot analysis of CEA using anti-CEA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates, Lane 9: mouse SP20 whole cell lysates, Lane 10: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
CEACAM5 / CD66e Antibody LS‑C313470
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Product
CEACAM5 / CD66e Antibody (PE) LS‑C485366
Species: Human
Applications: Flow Cytometry
See all CEACAM5 / CD66e Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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