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Catalog Number Size Price
LS-C313470-10 10 µg $318 
LS-C313470-100 100 µg (0.5 mg/ml) $470 
CEACAM5 / CD66e Antibody - Western blot analysis of CEA using anti-CEA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates, Lane 9: mouse SP20 whole cell lysates, Lane 10: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
CEACAM5 / CD66e Antibody - WB of CEACAM5 / CD66e antibody. Lane 1: Recombinant Human CEA Protein 5ng. Lane 2: Recombinant Human CEA Protein 2.5ng. Lane 3: Recombinant Human CEA Protein 1.25ng. Lane 4: SW620 Cell Lysate.
CEACAM5 / CD66e Antibody - Western blot analysis of CEA using anti-CEA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates, Lane 9: mouse SP20 whole cell lysates, Lane 10: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
CEACAM5 / CD66e Antibody - WB of CEACAM5 / CD66e antibody. Lane 1: Recombinant Human CEA Protein 5ng. Lane 2: Recombinant Human CEA Protein 2.5ng. Lane 3: Recombinant Human CEA Protein 1.25ng. Lane 4: SW620 Cell Lysate.
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Polyclonal Rabbit anti‑Human CEACAM5 / CD66e Antibody (IHC, WB) LS‑C313470

Polyclonal Rabbit anti‑Human CEACAM5 / CD66e Antibody (IHC, WB) LS‑C313470

Note: This antibody replaces LS-C388243
Antibody:
CEACAM5 / CD66e Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313470-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CEACAM5 / CD66e Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD66e antibody LS-C313470 is an unconjugated rabbit polyclonal antibody to human CD66e (CEACAM5). Validated for ELISA, IHC and WB.
Target
Human CEACAM5 / CD66e
Synonyms
CEACAM5 | CD66e | CD66e antigen | CEA | Carcinoembryonic antigen | Meconium antigen 100
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
Insect cells-derived human CEA recombinant protein(Position: full).
Specificity
Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CEACAM5 / CD66e
P06731 NM_004363 NP_004354.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024