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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BUB3

BUB3 mitotic checkpoint protein

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Gene Name: BUB3 mitotic checkpoint protein
Synonyms: BUB3, Budding uninhibited by benomyl, HBUB3, BUB3L, Mitotic checkpoint component
Target Sequences: NM_004725 NP_004716.1 O43684

Filtered By: None
Target
BUB3 (27)
Reactivity
Human (26)
Mouse (17)
Rat (14)
Bovine (8)
Chimpanzee (3)
Dog (2)
Drosophila melanogaster (1)
Guinea pig (8)
Hamster (4)
Horse (8)
Monkey (6)
Orangutan (3)
Pig (6)
Rabbit (8)
Sheep (1)
Application
IHC (12)
IHC-Fr (1)
IHC-P (8)
WB (22)
ELISA (11)
ICC (3)
IF (9)
IP (1)
Peptide-ELISA (1)
Host
rabbit (25)
mouse (1)
goat (1)
Isotype
Ig (1)
IgG (15)
IgG2b,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (26)
Clone
AT2H6 (1)
Format
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (21)
Epitope
N-Terminus (5)
C-Terminus (2)
aa275-325 (2)
260-340 aa, C-terminal (1)
His274 (1)
aa 201-230 (1)
aa1-328 (1)
aa1-50 (1)
aa279-328 (1)
aa76-90 (1)
Publications
No (27)
Showing 1-25 of 27 results

BUB3 Antibody (C-Terminus) IHC-plus™ LS-B1340
Rabbit Polyclonal (IgG) to Human BUB3
Mouse, Human
ICC IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-BUB3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 ug/ml.
Anti-BUB3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 ug/ml.

BUB3 Antibody (C-Terminus) LS-C353144
Rabbit Polyclonal to Human BUB3
Human, Monkey
ICC IF IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of BUB3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of BUB3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

BUB3 Antibody (aa76-90) LS-C312796
Rabbit Polyclonal to Human BUB3
Rabbit, Mouse, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Human, Monkey
ICC IHC IHC-Fr IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $355.00
BUB3 antibody. IHC(P): Rat Testis Tissue.
BUB3 antibody. IHC(P): Human Breast Cancer Tissue.
BUB3 antibody. IHC(P): Rat Testis Tissue.
BUB3 antibody. IHC(P): Human Breast Cancer Tissue.

BUB3 Antibody IHC-plus™ LS-B4858
Rabbit Polyclonal to Human BUB3
Human
IHC IHC-P WB
Unconjugated
100µl 0.84mg/ml  $425.00
Anti-BUB3 antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BUB3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BUB3 antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BUB3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

BUB3 Antibody (260-340 aa, C-terminal) LS-C382332
Rabbit Polyclonal (IgG) to Human BUB3
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Bub3 antibody
Western blot of Bub3 antibody
Western blot of Bub3 antibody
Western blot of Bub3 antibody

BUB3 Antibody (His274) LS-C176965
Rabbit Polyclonal to Human BUB3
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of Bub3 (H274) pAb in paraffin-embedded human brain tissue.
Western blot of Bub3 (H274) pAb in extracts from HeLa cells.
IHC of Bub3 (H274) pAb in paraffin-embedded human brain tissue.
Western blot of Bub3 (H274) pAb in extracts from HeLa cells.

BUB3 Antibody (aa275-325) LS-C287260
Rabbit Polyclonal (IgG) to Human BUB3
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Horse, Human
IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $405.00
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:1000 (1 ug/ml).
Detection of Human BUB3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-BUB3 antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:1000 (1 ug/ml).
Detection of Human BUB3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-BUB3 antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.

BUB3 Antibody (aa1-50) LS-C287258
Rabbit Polyclonal (IgG) to Human BUB3
Rabbit, Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Human
IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $405.00
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:1000 (1 ug/ml).
Detection of Human BUB3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-BUB3 antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:1000 (1 ug/ml).
Detection of Human BUB3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-BUB3 antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.

BUB3 Antibody LS-C334784
Rabbit Polyclonal (IgG) to Human BUB3
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded Rat kidney tissue.
Immunofluorescence analysis of U20S cells.
Immunohistochemistry of paraffin-embedded Rat kidney tissue.
Immunofluorescence analysis of U20S cells.

BUB3 Antibody LS-C498467
Rabbit Polyclonal (IgG) to Human BUB3
Mouse, Human
ELISA IHC WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human prostate cancer tissue using BUB3 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colonic cancer tissue using BUB3 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human prostate cancer tissue using BUB3 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colonic cancer tissue using BUB3 Antibody at dilution of 1:100

BUB3 Antibody (HRP) LS-C498456
Rabbit Polyclonal (IgG) to Human BUB3
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

BUB3 Antibody (Biotin) LS-C498460
Rabbit Polyclonal (IgG) to Human BUB3
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

BUB3 Antibody (FITC) LS-C498469
Rabbit Polyclonal (IgG) to Human BUB3
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00

BUB3 Antibody (aa275-325) LS-C286392
Rabbit Polyclonal (IgG) to Human BUB3
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Horse, Human
IF IHC IHC-P
Unconjugated
100µl  $415.00
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:250.
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:250.
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:250.
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:250.

BUB3 Antibody (N-Terminus) LS-C136992
Rabbit Polyclonal (IgG) to Human BUB3
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl  $255.00

BUB3 Antibody LS-C659226
Rabbit Polyclonal (IgG) to Human BUB3
Human
ELISA IF
Unconjugated
100µg  $435.00

BUB3 Antibody (clone AT2H6) LS-C755408
Mouse Monoclonal [clone AT2H6] (IgG2b,k) to Human BUB3
Human
ELISA WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $315.00
The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

BUB3 Antibody LS-C405133
Rabbit Polyclonal (IgG) to Human BUB3
Mouse, Human
ELISA WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Western blot analysis of Jurkat and HeLa cell, using BUB3 Polyclonal Antibody at dilution of 1:300.
Western blot analysis of Jurkat and HeLa cell, using BUB3 Polyclonal Antibody at dilution of 1:300.

BUB3 Antibody (aa1-328) LS-C125210
Goat Polyclonal (IgG) to Human BUB3
Human
ELISA WB
Unconjugated
100µg  $770.00

BUB3 Antibody (aa 201-230) LS-C757986
Rabbit Polyclonal (Ig) to Drosophila melanogaster BUB3
Drosophila melanogaster
ELISA WB
Unconjugated
80µl  $215.00; 400µl  $325.00
Western blot analysis of lysate from fruit fly, using BUB3 Antibody (Drosophila). BUB3 Antibody (Drosophila) was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot analysis of lysate from fruit fly, using BUB3 Antibody (Drosophila). BUB3 Antibody (Drosophila) was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

BUB3 Antibody (aa279-328) LS-C119398
Rabbit Polyclonal (IgG) to Human BUB3
Mouse, Rat, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using BUB3 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using BUB3 Antibody. The lane on the right is blocked with the synthesized peptide.

BUB3 Antibody LS-C417072
Rabbit Polyclonal to Human BUB3
Mouse, Rat, Human
WB
Unconjugated
100µg 1mg/ml  $305.00

BUB3 Antibody LS-C356834
Rabbit Polyclonal to Human BUB3
Human
WB
Unconjugated
100µl  $305.00
All lanes : Anti-BUB3 Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-BUB3 Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

BUB3 Antibody (N-Terminus) LS-C112219
Rabbit Polyclonal to Human BUB3
Rabbit, Mouse, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
WB
Unconjugated
50µg  $365.00
BUB3 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
BUB3 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

BUB3 Antibody (N-Terminus, Biotin) LS-C465245
Rabbit Polyclonal to Human BUB3
Rabbit, Mouse, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Orangutan, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
BUB3 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
BUB3 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 27 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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