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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCAR3

Breast Cancer Anti-estrogen Resistance 3

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: Breast Cancer Anti-estrogen Resistance 3
Synonyms: BCAR3, NSP2, SH2D3B, Novel SH2-containing protein 2
Target Sequences: NM_003567 NP_003558.1 O75815

Filtered by: None
Target
BCAR3 (21)
Reactivity
Human (20)
Mouse (5)
Rat (4)
Bovine (1)
Dog (2)
Monkey (1)
Application
IHC (7)
IHC-P (4)
WB (19)
ELISA (5)
ICC (1)
IF (4)
IP (1)
Peptide-ELISA (1)
Host
rabbit (19)
mouse (1)
goat (1)
Isotype
IgG (6)
IgG2a,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (20)
Clone
3G4 (1)
Format
APC (1)
APC Conjugated (1)
Biotin Conjugated (1)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
PE Conjugated (1)
Unconjugated (14)
Epitope
aa548-818 (8)
730-810 aa, C-terminal (1)
C-Terminus (1)
aa25-75 (1)
aa785-818 (1)
aa791-825 (1)
aa813-825 (1)
Publications
No (21)
Showing 1-21 of 21 results

IHC-plus™ BCAR3 Antibody LS-B7811
Rabbit Polyclonal to Human BCAR3
Human
IHC, IHC-P
Unconjugated
50µg 1mg/ml  $425.00
Anti-BCAR3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

BCAR3 Antibody LS-C800568
Rabbit Polyclonal (IgG) to Human BCAR3
Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

BCAR3 Antibody (C-Terminus) LS-C413099
Rabbit Polyclonal to Human BCAR3
Bovine, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of BCAR3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of BCAR3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

BCAR3 Antibody (730-810 aa, C-terminal) LS-C382249
Rabbit Polyclonal (IgG) to Human BCAR3
Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

BCAR3 Antibody (aa791-825) LS-C408057
Rabbit Polyclonal to Human BCAR3
Mouse, Dog, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µg  $355.00
BCAR3 was detected in paraffin-embedded sections of mouse lymphadenoma tissues using rabbit anti- BCAR3 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
BCAR3 was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- BCAR3 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

BCAR3 Antibody LS-C490518
Rabbit Polyclonal (IgG) to Human BCAR3
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µg  $435.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

BCAR3 Antibody LS-C153636
Rabbit Polyclonal (IgG) to Human BCAR3
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
0.1mg 1mg/ml  $365.00
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.

BCAR3 Antibody LS-C416170
Rabbit Polyclonal to Human BCAR3
Human
IHC, WB
Unconjugated
100µg  $305.00
Western blot of HeLa cell lysate using BCAR3 Antibody

BCAR3 Antibody (clone 3G4) LS-C198558
Mouse Monoclonal [clone 3G4] (IgG2a,k) to Human BCAR3
Human
ELISA, WB
Unconjugated
100µg  $365.00
Detection limit for recombinant GST tagged BCAR3 is approximately 0.03 ng/ml as a capture antibody.

BCAR3 Antibody (aa25-75) LS-C288459
Rabbit Polyclonal (IgG) to Human BCAR3
Human
IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00

BCAR3 Antibody (aa813-825) LS-C54573
Goat Polyclonal to Human BCAR3
Mouse, Dog, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
BCAR3 antibody (0.5 ug/ml) staining of Mouse Kidney epithelial cells lysate (15 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BCAR3 Antibody (aa785-818) LS-C344659
Rabbit Polyclonal to Mouse BCAR3
Mouse
WB
Unconjugated
400µl  $325.00
Western blot of lysates from mouse spleen tissue lysate with MOUSE Bcar3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

BCAR3 Antibody LS-C793370
Rabbit Polyclonal (IgG) to Human BCAR3
Human
ELISA
Unconjugated
100µg 1.3mg/ml  $365.00
ELISA: BCAR3 Antibody

BCAR3 Antibody (aa548-818) LS-C373502
Rabbit Polyclonal to Human BCAR3
Human
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00
Western blot of BCAR3 antibody.

BCAR3 Antibody (aa548-818, HRP) LS-C713713
Rabbit Polyclonal to Human BCAR3
Human
WB
HRP Conjugated
100µl 0.5mg/ml  $285.00; 200µl 0.5mg/ml  $345.00; 1ml 0.5mg/ml  $680.00
Western blot of BCAR3 antibody.

BCAR3 Antibody (aa548-818, Biotin) LS-C688824
Rabbit Polyclonal to Human BCAR3
Human
WB
Biotin Conjugated
100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $575.00
Western blot of BCAR3 antibody.

BCAR3 Antibody (aa548-818, FITC) LS-C695842
Rabbit Polyclonal to Human BCAR3
Human
WB
FITC Conjugated
100µl 0.5mg/ml  $275.00; 200µl 0.5mg/ml  $325.00; 1ml 0.5mg/ml  $635.00
Western blot of BCAR3 antibody.

BCAR3 Antibody (aa548-818, APC, Cy7) LS-C736696
Rabbit Polyclonal to Human BCAR3
Human
WB
APC, Cy7 Conjugated
100µl 0.1mg/ml  $385.00; 200µl 0.1mg/ml  $475.00; 1ml 0.1mg/ml  $1,170.00
Western blot of BCAR3 antibody.

BCAR3 Antibody (aa548-818, Cy3) LS-C704698
Rabbit Polyclonal to Human BCAR3
Human
WB
Cy3 Conjugated
100µl 0.1mg/ml  $355.00; 200µl 0.1mg/ml  $445.00; 1ml 0.1mg/ml  $1,050.00
Western blot of BCAR3 antibody.

BCAR3 Antibody (aa548-818, PE) LS-C727781
Rabbit Polyclonal to Human BCAR3
Human
WB
PE Conjugated
100µl 0.1mg/ml  $245.00; 200µl 0.1mg/ml  $285.00; 1ml 0.1mg/ml  $475.00
Western blot of BCAR3 antibody.

BCAR3 Antibody (aa548-818, APC) LS-C741662
Rabbit Polyclonal to Human BCAR3
Human
WB
APC Conjugated
100µl 0.1mg/ml  $275.00; 200µl 0.1mg/ml  $315.00; 1ml 0.1mg/ml  $590.00
Western blot of BCAR3 antibody.
Showing 1-21 of 21 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).