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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCAR3 Antibody LS‑C153636

Catalog Number Size Price
LS-C153636-0.1 0.1 mg (1 mg/ml) $365 
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.
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2 of 2

BCAR3 Antibody LS‑C153636

Antibody:
Rabbit Polyclonal to Human BCAR3
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365 
LS-C153636-0.1
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BCAR3
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BCAR3 antibody LS-C153636 is an unconjugated rabbit polyclonal antibody to BCAR3 from human, mouse and rat. Validated for ELISA, IF and WB.
Target
Human BCAR3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Immunogen
a 16 amino acid synthetic peptide near the N-terminus of human BCAR3. The immunogen is located within amino acids 30 - 80 of BCAR3.
Blocking Peptide
LS-E31318 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-C153636. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.
Usage
BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 91 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BCAR3
O75815 NM_003567 NP_003558.1

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Immunohistochemical analysis of BCAR3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Bovine
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Anti-BCAR3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin
Immunohistochemistry - Paraffin Image
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
BCAR3 was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- BCAR3 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Species: Human, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Western blot
Reactivity: Human
Range: Positive/Negative

Requested From: United States
Date Requested: 8/22/2019
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