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| Catalog Number | Size | Price |
|---|---|---|
| LS-G137545-10 | 10 µg (0.2 mg/ml) | $306 |
| LS-G137545-25 | 25 µg (0.2 mg/ml) | $367 |
| LS-G137545-100 | 100 µg | $689 |
| LS-G137545-500 | 500 µg | $1,577 |
Mouse TNF Alpha Protein (Recombinant) - LS-G137545
Mouse TNF Alpha Protein (Recombinant) - LS-G137545
Description:
TNF Alpha Protein LS-G137545 is a Recombinant Mouse TNF Alpha. It is low in endotoxin; Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method). For Research Use Only
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Description:
TNF Alpha Protein LS-G137545 is a Recombinant Mouse TNF Alpha. It is low in endotoxin; Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Mouse
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 156 amino acid recombinant protein has a predicted molecular mass of 17,257 Da. The DTT-reduced and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Leu.
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D. The ED50 for this effect is 0.5 €“ 3 pg/mL.
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 7.2, 10 mM NaH2PO4, 150 mM NaCl
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
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Images
Cytotoxic Assay
Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D.
Cytotoxic Assay
Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D.
Cytotoxic Assay
Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D.
Cytotoxic Assay
Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D.
Popular TNF Alpha Proteins
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
His, N-Terminal
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/4/2024
Date Requested: 5/4/2024
