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Catalog Number Size Price
LS-G259-10 10 µg $268 
LS-G259-50 50 µg $349 
Protein

Human TNF Alpha Protein (Recombinant) - LS-G259

Human TNF Alpha Protein (Recombinant) - LS-G259

Description:
TNF Alpha Protein LS-G259 is a Recombinant Human TNF Alpha produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only
Price
Catalog Number
$268
LS-G259-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
TNF Alpha Protein LS-G259 is a Recombinant Human TNF Alpha produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only

Specifications

Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Human
Modifications
Unmodified. Also available Animal-free.
Conjugations
Unconjugated
Predicted Molecular Weight
17.4 kDa
AA Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is <=0.05 ng/ml, corresponding to a specific activity of >=2 x 10^7 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 3 mM Sodium Phosphate, pH 8.0 + 20mM NaCl.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 2 hours before use. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/28/2024