Target
IL-10
Synonyms
IL10 | CSIF | GVHDS | IL-10 | IL10A | Interleukin-10 | Interleukin 10 | TGIF
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 161 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 18.9 kDa. The DTT-reduced protein migrates at approximately 18kDa and non-reduced protein migrates at approximately 15kDa by SDS-PAGE.
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
ED50 is equal to0.1- 0.5 ng/ml, corresponding to a specific activity of 2- 10 x 10^6 units/mg, as determined by the dose dependent stimulation MC/9 cell proliferation.
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution in 20 mM Tris, pH 7.5
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee