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Catalog Number Size Price
LS-B15330-50 50 µl (3.06 mg/ml) $460 
B2M / Beta 2 Microglobulin Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
B2M / Beta 2 Microglobulin Antibody - Immunofluorescence analysis of MCF-7 cells using B2M antibody. Blue: DAPI for nuclear staining.
B2M / Beta 2 Microglobulin Antibody - Western blot analysis of extracts of various cell lines, using B2M antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
B2M / Beta 2 Microglobulin Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
B2M / Beta 2 Microglobulin Antibody - Immunofluorescence analysis of MCF-7 cells using B2M antibody. Blue: DAPI for nuclear staining.
B2M / Beta 2 Microglobulin Antibody - Western blot analysis of extracts of various cell lines, using B2M antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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PathPlus™ Polyclonal Rabbit anti‑Human B2M / Beta 2 Microglobulin Antibody (IHC, IF, WB) LS‑B15330

PathPlus™ Polyclonal Rabbit anti‑Human B2M / Beta 2 Microglobulin Antibody (IHC, IF, WB) LS‑B15330

Note: This antibody replaces LS-C331544
Antibody:
B2M / Beta 2 Microglobulin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B15330-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
B2M / Beta 2 Microglobulin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
B2M / Beta 2 Microglobulin is a serum protein associated with major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. It is required for proper cell surface expression of the complex. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. In cancer, mutation, loss or deregulation of B2M is believed to be involved in aiding tumors in escaping the immune response and therapies. It is used as a marker in the diagnosis of multiple myeloma and lymphoma, where it is regularly overexpressed. It has elevated levels in a number of other cancers, including colorectal, breast, gastric, lung, prostate and testicular cancer as well as malignant epithelial ovarian tumors. B2M has membranous and cytoplasmic positivity in most normal tissues, with elevated levels in immune cells and macrophages. References: J Transl Med. 2016; 14: 75, PMID: 26983758; Cancer Discov. 2017 Dec; 7(12): 1420–1435, PMID: 29025772
Target
Human B2M / Beta 2 Microglobulin
Synonyms
B2M | Beta 2 Microglobulin | Beta-2-microglobulin | Beta-2-microglobin
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human B2M (NP_004039.1). IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Specificity
Human B2M / Beta 2 Microglobulin
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 13kDa, while the observed MW by Western blot was 12kDa.
Presentation
PBS, 50% glycerol, pH 7.3
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About B2M / Beta 2 Microglobulin
P61769 NM_004048 NP_004039.1

Validation

B2M / Beta 2 Microglobulin Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.

LSBio Ratings

PathPlus™ B2M / Beta 2 Microglobulin Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B15330 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/25/2024