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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B2200-100 100 µg (0.5 mg/ml) $425 
B2M / Beta 2 Microglobulin Antibody - Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
B2M / Beta 2 Microglobulin Antibody - Human peripheral blood lymphocytes stained with purified 2M2, followed by anti-mouse FITC.
B2M / Beta 2 Microglobulin Antibody - Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
B2M / Beta 2 Microglobulin Antibody - Human peripheral blood lymphocytes stained with purified 2M2, followed by anti-mouse FITC.
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PathPlus™ B2M / Beta 2 Microglobulin Antibody Monoclonal ELISA,Flo,IHC,WB LS‑B2200

PathPlus™ B2M / Beta 2 Microglobulin Antibody Monoclonal ELISA,Flo,IHC,WB LS‑B2200

Note: This antibody replaces LS-C41051
Antibody:
Mouse Monoclonal to Human B2M / Beta 2 Microglobulin
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2200-100
100 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human B2M / Beta 2 Microglobulin
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
B2M / Beta 2 Microglobulin is a serum protein associated with major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. It is required for proper cell surface expression of the complex. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. In cancer, mutation, loss or deregulation of B2M is believed to be involved in aiding tumors in escaping the immune response and therapies. It is used as a marker in the diagnosis of multiple myeloma and lymphoma, where it is regularly overexpressed. It has elevated levels in a number of other cancers, including colorectal, breast, gastric, lung, prostate and testicular cancer as well as malignant epithelial ovarian tumors. B2M has membranous and cytoplasmic positivity in most normal tissues, with elevated levels in immune cells and macrophages. J Transl Med. 2016; 14: 75, PMID: 26983758; Cancer Discov. 2017 Dec; 7(12): 1420–1435, PMID: 29025772
Target
Human B2M / Beta 2 Microglobulin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
2M2
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified human B2M / Beta-2-Microglobulin.
Specificity
Purified human beta-2-microglobulin.
Usage
Immunohistochemistry: LS-B2200 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2200 was determined to be 5 ug/ml.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Storage
Store at 4°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About B2M / Beta 2 Microglobulin
P61769 NM_004048 NP_004039.1

Validation

B2M / Beta 2 Microglobulin Antibody - Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Publications (3)

NLR family member NLRC5 is a transcriptional regulator of MHC class I genes. Meissner TB, Li A, Biswas A, Lee KH, Liu YJ, Bayir E, Iliopoulos D, van den Elsen PJ, Kobayashi KS. Proceedings of the National Academy of Sciences of the United States of America. 2010 107:13794-9.

Distinct functions for the glycans of tapasin and heavy chains in the assembly of MHC class I molecules. Rizvi SM, Del Cid N, Lybarger L, Raghavan M. Journal of immunology (Baltimore, Md. : 1950). 2011 186:2309-20.

NLRC5 cooperates with the RFX transcription factor complex to induce MHC class I gene expression. Meissner TB, Liu YJ, Lee KH, Li A, Biswas A, van Eggermond MC, van den Elsen PJ, Kobayashi KS. Journal of immunology (Baltimore, Md. : 1950). 2012 188:4951-8.

Customer Reviews (0)


Requested From: United States
Date Requested: 11/12/2019