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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B5565-0.1 0.1 ml $300 
EPCAM Antibody - Anti-EPCAM antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
EPCAM Antibody - Anti-EPCAM antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
EPCAM Antibody - Anti-EPCAM antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
EPCAM Antibody - Anti-EPCAM antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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2 of 2

PathPlus™ EPCAM Antibody Monoclonal Concentrated IHC,WB LS‑B5565

PathPlus™ EPCAM Antibody Monoclonal Concentrated IHC,WB LS‑B5565

Note: This antibody replaces LS-C134852
Antibody:
Mouse Monoclonal to Human EPCAM
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Concentrated
Price
Catalog Number
$300 
LS-B5565-0.1
0.1 ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human EPCAM
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Concentrated

Specifications

Description
EPCAM (TACSTD1) is an epithelial membrane glycoprotein involved in cell adhesion that is present on the surface of a variety of epithelial cells. It is expressed in a wide variety of cancers, including: basal cell carcinomas, mammary Paget disease, lung adenocarcinomas, trichoepitheliomas, dermatofibromas, basal-cell carcinomas, cholangiocarcinomas, colorectal carcinomas, and carcinomas of prostate, ovary, endometrium, head and neck, and thyroid. EPCAM is useful for distinguishing epithelial cells (positive) from mesothelial cells (negative). It has also been implicated in the progression of individuals with Lynch Syndrome who hold inherited deletions in the gene. These deletions lead to silencing of the adjacent repair gene MSH2 via transcriptional read-through, which then causes microsatellite instability and colorectal cancer (as well as other malignancies that also frequently arise from Lynch Syndrome). MOC31 and BerEP4 are common monoclonal antibodies to this target. JClinPathol 1990,43:213; Acta Neuropathol 1991, 83:46; Dai, 2017; Baeuerle, 2007; Kempers 2010; ModPath 2002, 15:1279
Target
Human EPCAM
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
MOC-31
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Concentrated
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
This antibody is reactive with lung cancer associated antigens and has been studied and categorized in different clusters of reactivity patterns during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. MOC-31 reacts with most epithelia, and, in lung cancer, all lung carcinomas. The membrane-associated proteins detected by MOC-31 appear to have an apparent molecular weight of 35-40 kD. MOC-31 is not reactive with normal and malignant mesothelia and therefore is especially useful for the detection of carcinoma cells in ascites or pleural effusions (4).
Usage
The antibody is useful in immunohistochemistry and immunoblotting. MOC-31 react with antigens detectable in cryostat section. For use on frozen tissue and paraffin tissue after antigen retrieval. Using the antigen retrieval techniques this antibody discriminates between cells which originate from mesothelium and epithelium. General procedure to perform cryostat sectioning and immunostaining: Cryostat sectioning and immunostaining is done as described by de Leij et al. Sections (about 6 micron thick) are cut in a cryostat at -20°C and placed on glass slides. After drying at RT and fixation in acetone (water-free), the sections are washed 2-3 times in PBS. Subsequently (after drying the glass area around the sections) 25 ul of undiluted monoclonal antibody preparation is applied to the wet sections. After incubation for 45 mins. in a humidified atmosphere, the sections are washed again in PBS (2-3 times) and the second step reagent (an appropriately diluted HRPO-conjugated, anti mouse Ig preparation, supplemented with 1% human serum) is applied to the wet sections. After incubation for an additional 20 mins., the sections are washed again in PBS (2-3) times and the staining reaction is performed with 3-aminoethylcarbazole (10 mg dissolved in 2.5 ml dimethylformamide, subsequently 0.05 M acetate buffer, pH 4.9, is added to a total volume of 50 ml, after which the solution is filtrated and H2O2 is added to a final concentration of about 0.03%). A positive reaction is indicated by red deposit. The nuclei of the cells present in the sections are counterstained with Mayer's hematoxylin to obtain a good histological picture. Working dilution: approx 1:20.
Presentation
Tissue culture supernatant (8% FBS), <0.1% sodium azide
Storage
Store at 2-8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EPCAM
P16422 NM_002354 NP_002345.2

Validation

EPCAM Antibody - Anti-EPCAM antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-EPCAM antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-EPCAM antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

EPCAM Antibody - Anti-EPCAM antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-EPCAM antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-EPCAM antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

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Requested From: United States
Date Requested: 12/10/2019