Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Features: - Rabbit IgG primary antibody detection - Peroxidase enzyme detection system - Avidin/biotin complex (ABC) technology - Good sensitivity/low background - Used as a second label in multiple antigen labeling applications - Sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Kit Contents: - 3 ml Normal Goat Serum - 1 ml Biotinylated Goat Anti-Rabbit IgG - 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated Peroxidase) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® ABC-HRP Staining Kit (Peroxidase, Rabbit IgG) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated. This kit consists of the Blocking Serum, a species specific Biotinylated Secondary Antibody, and the HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol. Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1009). Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting