Features: - Peroxidase enzyme detection system - Avidin/biotin complex (ABC) technology - High sensitivity/low background - Used as a second label in multiple antigen labeling applications - CE Mark - No antibody included - Concentrate Kit Contents: - 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated HRP) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® ABC-HRP Staining Kit (Peroxidase, Standard) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated. This Standard kit consists of the HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol only, and is intended to be paired with biotinylated secondary antibodies, such as LS-D1 (goat anti-rabbit IgG), LS-D2 (horse anti-mouse IgG), or LS-D3 (horse anti-goat IgG). Alternatively, this kit is available pre-packaged with various combinations of species specific blocking serum and biotinylated secondary antibody (see VECTASTAIN® ABC-HRP Staining Kits LS-J1010, LS-J1011, LS-J1012, LS-J1013, LS-J1014, LS-J1015, LS-J1016, and LS-J1017) Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting