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Catalog Number Size Price
LS-C391163-20 20 µg (0.2 mg/ml) $354 
LS-C391163-100 100 µg (0.2 mg/ml) $550 
ZAP70 Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with ZAP70 antibody (SPM362).  This image was taken for the unmodified form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human ZAP70 Antibody (clone SPM362, IHC, IF) LS‑C391163

Monoclonal Mouse anti‑Human ZAP70 Antibody (clone SPM362, IHC, IF) LS‑C391163

Antibody:
ZAP70 Mouse anti-Human Monoclonal (SPM362) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$354
LS-C391163-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ZAP70 Mouse anti-Human Monoclonal (SPM362) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
ZAP70 antibody LS-C391163 is an unconjugated mouse monoclonal antibody to human ZAP70. Validated for Flow, IF and IHC.
Target
Human ZAP70
Synonyms
ZAP70 | 70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
SPM362
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free, BSA-free.
Immunogen
Recombinant human protein (including amino acids 1-254 and the SH2 domains).
Specificity
Human ZAP70
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis. Optimal dilution of the ZAP70 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ZAP70
P43403 NM_207519 P43403

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/25/2022