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Antibodies > ZAP70 > LS-C413113
 
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Catalog Number Size Price
LS-C413113-100 100 µl (1 mg/ml) $341 
ZAP70 Antibody - Immunohistochemical analysis of ZAP70 (pY319) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ZAP70 Antibody - Immunofluorescent analysis of ZAP70 (pY319) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ZAP70 Antibody - Western blot analysis of ZAP70 (pY319) expression in SHSY5Y (A); Jurkat (B) whole cell lysates.
ZAP70 Antibody - Immunohistochemical analysis of ZAP70 (pY319) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ZAP70 Antibody - Immunofluorescent analysis of ZAP70 (pY319) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ZAP70 Antibody - Western blot analysis of ZAP70 (pY319) expression in SHSY5Y (A); Jurkat (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human ZAP70 Antibody (phospho‑Tyr319, IHC, IF, WB) LS‑C413113

Polyclonal Rabbit anti‑Human ZAP70 Antibody (phospho‑Tyr319, IHC, IF, WB) LS‑C413113

Submit Review LSBio Guarantee
Antibody:
ZAP70 Rabbit anti-Human Polyclonal (pTyr319) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$341
LS-C413113-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ZAP70 Rabbit anti-Human Polyclonal (pTyr319) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ZAP70 antibody LS-C413113 is an unconjugated rabbit polyclonal antibody to ZAP70 (pTyr319) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.
Target
Human ZAP70
Synonyms
ZAP70 | 70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human ZAP70 (pY319).
Epitope
pTyr319
Specificity
Recognizes endogenous levels of ZAP70 (pY319) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ZAP70
P43403 NM_207519 P43403

Publications (0)

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ZAP70 Antibody (phospho‑Tyr493) LS‑C117511
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ZAP70 Antibody - Immunohistochemistry analysis of paraffin-embedded human lymph node, using ZAP-70 (Phospho-Tyr315) Antibody. The picture on the right is blocked with the phospho peptide.
ZAP70 Antibody (phospho‑Tyr315) LS‑C199744
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ZAP70 Antibody (aa301‑350) LS‑C151431
Species: Mouse
Applications: Western blot
ZAP70 ELISA Kit
Mouse/Human Phospho-ZAP70 (Tyr315) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1238
Reactivity: Human, Mouse
Range: Positive/Negative
ZAP70 ELISA Kit
Mouse/Human/Rat Phospho-ZAP70 (Tyr493) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1650
Reactivity: Human, Mouse, Rat
Range: Positive/Negative
ZAP70 ELISA Kit
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Reactivity: Human
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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