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Catalog Number Size Price
LS-C773628-100 100 µg (1 mg/ml) $477 
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
WDFY3 / ALFY Antibody - Western blot analysis of Rat brain showing detection of 395.2 kDa WDFY3 protein using Rabbit Anti-WDFY3 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 395.2 kDa.
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
WDFY3 / ALFY Antibody - Western blot analysis of Rat brain showing detection of 395.2 kDa WDFY3 protein using Rabbit Anti-WDFY3 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 395.2 kDa.
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Polyclonal Rabbit anti‑Human WDFY3 / ALFY Antibody (Biotin, IF, WB) LS‑C773628

Polyclonal Rabbit anti‑Human WDFY3 / ALFY Antibody (Biotin, IF, WB) LS‑C773628

Antibody:
WDFY3 / ALFY Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IF, WB
Reactivity:
Human, Rat
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$477
LS-C773628-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
WDFY3 / ALFY Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IF, WB
Reactivity:
Human, Rat
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
ALFY antibody LS-C773628 is a biotin-conjugated rabbit polyclonal antibody to ALFY (WDFY3) from human. It is reactive with human and rat. Validated for IF and WB.
Target
Human WDFY3 / ALFY
Synonyms
WDFY3 | ALFY | Autophagy-linked FYVE protein | KIAA0993 | ZFYVE25
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 594, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of Human WDFY3 (aa. 533-544)
Specificity
Detects ~400 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WDFY3 / ALFY
Q8IZQ1 AB023210 BAA76837.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024