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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WDFY3 / ALFY

WD repeat and FYVE domain containing 3

Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation.

Gene Name: WD repeat and FYVE domain containing 3
Synonyms: WDFY3, ALFY, Autophagy-linked FYVE protein, KIAA0993, ZFYVE25
Target Sequences: AB023210 BAA76837.2 Q8IZQ1

Filtered By: None
Target
WDFY3 / ALFY (14)
Reactivity
Human (13)
Mouse (6)
Rat (5)
Bovine (2)
Chimpanzee (3)
Dog (2)
Gibbon (3)
Guinea pig (4)
Monkey (4)
Pig (6)
Rabbit (4)
Application
IHC (5)
IHC-P (3)
WB (7)
ELISA (3)
IF (3)
IP (1)
Peptide-ELISA (1)
Host
rabbit (12)
mouse (1)
goat (1)
Isotype
IgG (11)
IgG1,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (13)
Clone
2F12 (1)
Format
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (8)
Epitope
aa506-555 (4)
aa500-550 (2)
aa2750-2800 (1)
internal region (1)
Publications
No (14)
Showing 1-14 of 14 results

WDFY3 / ALFY Antibody (aa500-550) IHC-plus™ LS-B12067
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Dog, Bovine, Pig, Human
IF IHC IHC-P IP WB
Unconjugated
50µl 1mg/ml  $425.00
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

WDFY3 / ALFY Antibody LS-C672676
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Human
ELISA IF IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image of paraffin-embedded human colon cancer at a dilution of 1:100
Immunohistochemistry image of paraffin-embedded human gastric cancer at a dilution of 1:100
Immunohistochemistry image of paraffin-embedded human colon cancer at a dilution of 1:100
Immunohistochemistry image of paraffin-embedded human gastric cancer at a dilution of 1:100

WDFY3 / ALFY Antibody (HRP) LS-C672677
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Human
HRP Conjugated
50µg  $285.00; 100µg  $385.00

WDFY3 / ALFY Antibody (Biotin) LS-C672680
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Human
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

WDFY3 / ALFY Antibody (FITC) LS-C672679
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Human
FITC Conjugated
50µg  $285.00; 100µg  $385.00

WDFY3 / ALFY Antibody (aa500-550) LS-C286609
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Dog, Bovine, Pig, Human
IF IHC IHC-P
Unconjugated
100µl  $415.00
Detection of Human WDFY3 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-WDFY3 used at a dilution of 1:250.
Detection of Human WDFY3 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-WDFY3 used at a dilution of 1:250.
Detection of Human WDFY3 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-WDFY3 used at a dilution of 1:250.
Detection of Human WDFY3 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-WDFY3 used at a dilution of 1:250.

WDFY3 / ALFY Antibody LS-C754648
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Human
ELISA IHC
Unconjugated
100µg  $355.00

WDFY3 / ALFY Antibody (clone 2F12) LS-C139264
Mouse Monoclonal [clone 2F12] (IgG1,k) to Human WDFY3 / ALFY
Human
ELISA WB
Unconjugated
100µg  $355.00

WDFY3 / ALFY Antibody (internal region) LS-C742563
Goat Polyclonal to Mouse WDFY3 / ALFY
Mouse
Peptide-ELISA WB
Unconjugated
100µg  $405.00
Antibody (5µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (5µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WDFY3 / ALFY Antibody (aa2750-2800) LS-C483076
Rabbit Polyclonal to Human WDFY3 / ALFY
Mouse, Rat, Human
IHC
Unconjugated
100µl  $325.00
Rabbit antibody to WDFY3 (2750-2800). IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to WDFY3 (2750-2800). IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

WDFY3 / ALFY Antibody (aa506-555) LS-C82642
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Rabbit, Mouse, Guinea pig, Rat, Pig, Human, Monkey
WB
Unconjugated
50µg  $365.00
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

WDFY3 / ALFY Antibody (aa506-555, HRP) LS-C454308
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Rabbit, Mouse, Guinea pig, Rat, Pig, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

WDFY3 / ALFY Antibody (aa506-555, Biotin) LS-C454306
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Rabbit, Mouse, Guinea pig, Rat, Pig, Gibbon, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

WDFY3 / ALFY Antibody (aa506-555, FITC) LS-C454307
Rabbit Polyclonal (IgG) to Human WDFY3 / ALFY
Rabbit, Mouse, Guinea pig, Rat, Pig, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-14 of 14 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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