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WDFY3 / ALFY

WD repeat and FYVE domain containing 3

Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation.

Gene Name: WD repeat and FYVE domain containing 3
Synonyms: WDFY3, ALFY, Autophagy-linked FYVE protein, KIAA0993, ZFYVE25
Target Sequences: AB023210 BAA76837.2 Q8IZQ1

Publications (1)

1
ALFY localizes to early endosomes and cellular protrusions to facilitate directional cell migration. Kristiane Søreng, Serhiy Pankiv, Camilla Bergsmark, Ellen M Haugsten, Anette K Dahl, Laura R de la Ballina, Ai Yamamoto, Alf H Lystad, Anne Simonsen. Journal of cell science. 2022 February;135:jcs259138. [Full Text Article] [PubMed:35099014] [PMC:PMC8919339] Related Antibodies: LS-C483076.

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Antibodies (24)
Primary (24)
WDFY3 / ALFY (24)
Human (23)
Mouse (5)
Rat (14)
Chimpanzee (3)
Gibbon (3)
Guinea pig (3)
Monkey (3)
Pig (3)
Rabbit (3)
IHC (5)
IHC-P (2)
WB (17)
ELISA (3)
IF (11)
IP (1)
Peptide-ELISA (1)
rabbit (22)
mouse (1)
goat (1)
Product Group
IHCPlus (1)
IgG (11)
IgG1,k (1)
monoclonal mc (1)
polyclonal pc (23)
2F12 (1)
AP Conjugated (1)
APC Conjugated (1)
Atto 390 Conjugated (1)
Atto 488 Conjugated (1)
Atto 594 Conjugated (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (2)
PerCP Conjugated (1)
RPE Conjugated (1)
Unconjugated (9)
aa506-555 (4)
aa500-550 (2)
Internal Region (1)
aa2750-2800 (1)
No (23)
Yes (1)
WDFY3 / ALFY Antibody - Rabbit antibody to WDFY3 (2750-2800). IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
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WDFY3 / ALFY Antibody (aa2750-2800) LS-C483076
(4.0) Rating
(1) Publication(s)
WDFY3 / ALFY Rabbit anti-Human Polyclonal (aa2750-2800) Antibody
Mouse, Rat, Human
IHC
Unconjugated
100 µl/$393
WDFY3 / ALFY Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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IHC-plus™ WDFY3 / ALFY Antibody (aa500-550) LS-B12067
(4.0) Rating
WDFY3 / ALFY Rabbit anti-Human Polyclonal (aa500-550) Antibody
Human
IHC, IHC-P, IP, WB
Unconjugated
50 µl/$460
WDFY3 / ALFY Antibody - Immunohistochemistry image of paraffin-embedded human colon cancer at a dilution of 1:100
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WDFY3 / ALFY Antibody LS-C672676
WDFY3 / ALFY Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
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WDFY3 / ALFY Antibody (HRP) LS-C672677
WDFY3 / ALFY Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
WDFY3 / ALFY Antibody (Biotin) LS-C672680
WDFY3 / ALFY Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
Biotin Conjugated
50 µg/$294; 100 µg/$360
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WDFY3 / ALFY Antibody (FITC) LS-C672679
WDFY3 / ALFY Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
Select
WDFY3 / ALFY Antibody LS-C754648
WDFY3 / ALFY Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC
Unconjugated
100 µg/$341
Select
WDFY3 / ALFY Antibody (clone 2F12) LS-C139264
WDFY3 / ALFY Mouse anti-Human Monoclonal (2F12) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$423
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
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WDFY3 / ALFY Antibody LS-C773625
WDFY3 / ALFY Rabbit anti-Human Polyclonal Antibody
Rat, Human
IF, WB
Unconjugated
100 µg/$442
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
Select
WDFY3 / ALFY Antibody (Biotin) LS-C773628
WDFY3 / ALFY Rabbit anti-Human Polyclonal (Biotin) Antibody
Rat, Human
IF, WB
Biotin Conjugated
100 µg/$477
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
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WDFY3 / ALFY Antibody (FITC) LS-C773641
WDFY3 / ALFY Rabbit anti-Human Polyclonal (FITC) Antibody
Rat, Human
IF, WB
FITC Conjugated
100 µg/$473
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
Select
WDFY3 / ALFY Antibody (APC) LS-C773626
WDFY3 / ALFY Rabbit anti-Human Polyclonal (APC) Antibody
Rat, Human
IF, WB
APC Conjugated
100 µg/$479
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
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WDFY3 / ALFY Antibody (AP) LS-C773627
WDFY3 / ALFY Rabbit anti-Human Polyclonal (AP) Antibody
Rat, Human
IF, WB
AP Conjugated
100 µg/$474
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
Select
WDFY3 / ALFY Antibody (RPE) LS-C773631
WDFY3 / ALFY Rabbit anti-Human Polyclonal (RPE) Antibody
Rat, Human
IF, WB
RPE Conjugated
100 µg/$477
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
Select
WDFY3 / ALFY Antibody (PerCP) LS-C773630
WDFY3 / ALFY Rabbit anti-Human Polyclonal (PerCP) Antibody
Rat, Human
IF, WB
PerCP Conjugated
100 µg/$479
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
Select
WDFY3 / ALFY Antibody (Atto 488) LS-C773633
WDFY3 / ALFY Rabbit anti-Human Polyclonal (Atto 488) Antibody
Rat, Human
IF, WB
Atto 488 Conjugated
100 µg/$479
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
Select
WDFY3 / ALFY Antibody (Atto 390) LS-C773634
WDFY3 / ALFY Rabbit anti-Human Polyclonal (Atto 390) Antibody
Rat, Human
IF, WB
Atto 390 Conjugated
100 µg/$480
WDFY3 / ALFY Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
Select
WDFY3 / ALFY Antibody (Atto 594) LS-C773636
WDFY3 / ALFY Rabbit anti-Human Polyclonal (Atto 594) Antibody
Rat, Human
IF, WB
Atto 594 Conjugated
100 µg/$479
WDFY3 / ALFY Antibody - Antibody (5µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Select
WDFY3 / ALFY Antibody (Internal Region) LS-C742563
WDFY3 / ALFY Goat anti-Mouse Polyclonal (Internal Region) Antibody
Mouse
Peptide-ELISA, WB
Unconjugated
100 µg/$503
WDFY3 / ALFY Antibody - Detection of Human WDFY3 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-WDFY3 used at a dilution of 1:250.
Select
WDFY3 / ALFY Antibody (aa500-550) LS-C286609
WDFY3 / ALFY Rabbit anti-Human Polyclonal (aa500-550) Antibody
Human
IHC
Unconjugated
1 µg/$295; 100 µl/$532
WDFY3 / ALFY Antibody - Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
WDFY3 / ALFY Antibody (aa506-555) LS-C82642
WDFY3 / ALFY Rabbit anti-Human Polyclonal (aa506-555) Antibody
Human
WB
Unconjugated
100 µl/$424
WDFY3 / ALFY Antibody - Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
WDFY3 / ALFY Antibody (aa506-555, HRP) LS-C454308
WDFY3 / ALFY Rabbit anti-Human Polyclonal (aa506-555) (HRP) Antibody
Rabbit, Mouse, Guinea pig, Rat, Pig, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100 µl/$469
WDFY3 / ALFY Antibody - Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
WDFY3 / ALFY Antibody (aa506-555, Biotin) LS-C454306
WDFY3 / ALFY Rabbit anti-Human Polyclonal (aa506-555) (Biotin) Antibody
Rabbit, Mouse, Guinea pig, Rat, Pig, Gibbon, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
WDFY3 / ALFY Antibody - Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
WDFY3 / ALFY Antibody (aa506-555, FITC) LS-C454307
WDFY3 / ALFY Rabbit anti-Human Polyclonal (aa506-555) (FITC) Antibody
Rabbit, Mouse, Guinea pig, Rat, Pig, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100 µl/$469
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