Human VEGFA / VEGF

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Monoclonal

R010

Unconjugated

Protein A purified

Preservative Free

Recombinant Human VEGF165/VEGFA Protein

Human VEGFA. Has cross-reactivity with mouse VEGF164 and rat VEGF164 in ELISA assay

• ELISA (1 µg/ml)
• Blocking (5 µg/ml)
• Neutralization (10 ng/ml)

Block: In a functional ELISA which immobilized recombinant Human VEGF165 at 1 ug/ml (100 µL/well) in the plate, the Rabbit anti-Human VEGF165 Monoclonal Antibody can block the binding of 5 ug/ml of biotinylated recombinant Human VEGFR2 to human VEGF165, the EC50 is 1. 51 ug/ml. Neutralization: The neutralization activity of VEGF Neutralizing Antibody is measured by its ability to neutralize VEGF165 induced proliferation in HUVEC human umbilical vein endothelial cells. The VEGF Neutralization titer (IC50) is typically 5. 35-21. 4 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165. Please Note: Optimal concentrations/dilutions should be determined by the end user.

0.2 µm filtered solution in PBS

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15692 NCBI: NM_003376 NP_003367.4