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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C104581-100 100 µg $325 
VEGFA / VEGF Antibody - Western Blot (reducing) of VEGF antibody
VEGFA / VEGF Antibody - Western Blot (non-reducing) of VEGF antibody
VEGFA / VEGF Antibody - Sandwich ELISA of VEGF antibody
VEGFA / VEGF Antibody - Western Blot (reducing) of VEGF antibody
VEGFA / VEGF Antibody - Western Blot (non-reducing) of VEGF antibody
VEGFA / VEGF Antibody - Sandwich ELISA of VEGF antibody
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Polyclonal Rabbit anti‑Human VEGFA / VEGF Antibody (WB) LS‑C104581

Polyclonal Rabbit anti‑Human VEGFA / VEGF Antibody (WB) LS‑C104581

Antibody:
VEGFA / VEGF Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$325
LS-C104581-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
VEGFA / VEGF Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
VEGF antibody LS-C104581 is an unconjugated rabbit polyclonal antibody to human VEGF (VEGFA). Validated for ELISA and WB.
Target
Human VEGFA / VEGF
Synonyms
VEGFA | VPF | Vascular permeability factor | VEGF | VEGF-A | MVCD1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human VEGF165.
Specificity
Human VEGF
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5-5.5 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hVEGF (10 ng/ml), a concentration of 0.05-0.1 ug/ml of this antibody is required. ELISA: To detect hVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human VEGF (LS-C104615) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Centrifuge vial prior to opening. Reconstitiute in sterile water to 0.1-1.0 mg/ml. Do not vortex.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VEGFA / VEGF
P15692 NM_003376 NP_003367.4

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Requested From: United States
Date Requested: 9/22/2020