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| Catalog Number | Size | Price |
|---|---|---|
| LS-C126456-100 | 100 µg | $778 |
Monoclonal Mouse V5 Tag Antibody (HRP, WB) LS‑C126456
Monoclonal Mouse V5 Tag Antibody (HRP, WB) LS‑C126456
Antibody:
V5 Tag Mouse Monoclonal (HRP) Antibody
Application:
WB, ELISA
Format:
Horseradish Peroxidase, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
V5 Tag Mouse Monoclonal (HRP) Antibody
Application:
WB, ELISA
Format:
Horseradish Peroxidase, Unmodified
Specifications
Description
V5 Tag antibody LS-C126456 is an HRP-conjugated mouse monoclonal antibody to V5 Tag. Validated for ELISA and WB.
Host
Mouse
Clonality
IgG2a
Monoclonal
Conjugations
Horseradish Peroxidase
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Paramyxovirus SV5, Simian-Virus 5.
Specificity
Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.
Applications
- Western blot (1:1000)
- ELISA
Usage
Western Blot:1:1000. ELISA. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.01% Thimerosal
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Virus
Applications:
IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Radioimmunoassay
![V5 Tag Antibody - Anti-V5 Epitope Tag Polyclonal Antibody - Western Blot. Anti-V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. This antibody was used at 1.0 ug/ml to detect 0.05 ug (lane 2) of full-length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS-PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code for 45 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/v5-tag-antibody-ls-c154221/81901_301500.jpg)
Species:
Applications:
ICC, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Virus
Applications:
Immunofluorescence, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
Date Requested: 4/29/2024
