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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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V5 Tag

V5 Tag, GKPIPNPLLGLDST, is from the C-terminal sequence of the P and V proteins of Simian Virus 5. V5 tag antibodies are used in many applications such as western blot, immunoprecipitation and immunofluorescence, and are a reliable means of purifying target proteins in many species. The V5 tag is often used alongside His-tags in affinity purification (PMID 24510596).

V5 Tag Target Details

Target Name: V5 Tag

Filtered by: None
Target
V5 Tag (112)
Reactivity
Human (4)
All species (2)
Rabbit (4)
Virus (13)
Application
IHC (13)
IHC-Fr (21)
WB (98)
Flo (9)
DB (1)
ELISA (86)
ICC (8)
IF (43)
IP (62)
Micro (1)
RIA (24)
Host
rabbit (25)
mouse (69)
goat (7)
hamster-armenian (1)
human (5)
rat (4)
sheep (1)
Isotype
Fab (2)
IgG (21)
IgG,k (2)
IgG1 (35)
IgG1,k (3)
IgG2,k (1)
IgG2a (16)
IgG2a,k (8)
IgG2b,k (2)
IgG2c,k (2)
IgG3,k (2)
IgG4,k (1)
Clonality
monoclonal mc (63)
polyclonal pc (32)
recombinant monoclonal rmc (17)
Clone
1H6 (1)
4C12E11 (2)
E10 (2)
KT90 (1)
Mix (1)
Q31 (1)
SV5-P-K (17)
SV5-Pk1 (5)
SV5-Pk2 (1)
SV5-Pk3 (1)
SV5-Pk4 (2)
SV5-Pk5 (2)
Format
AP Conjugated (9)
APC Conjugated (1)
Azide-free (1)
Biotin (NHS-LC) Conjugated (1)
Biotin Conjugated (9)
FITC Conjugated (13)
HiLyte Fluor 488 Conjugated (1)
HiLyte Fluor 555 Conjugated (1)
HiLyte Fluor 647 Conjugated (1)
HRP Conjugated (11)
PE Conjugated (6)
Unconjugated (59)
Epitope
V5 epitope tag (17)
aa95-108 (8)
V5 epitope (1)
Publications
No (112)
Showing 1-25 of 112 results

Mouse Anti-V5 Tag Secondary Antibody (clone SV5-Pk1) LS-C57305
Mouse Monoclonal [clone SV5-Pk1] (IgG2a) to Virus V5 Tag
Virus
ELISA, Flo, IF, IHC-Fr, IP, RIA, WB
Unconjugated
1000µg 1mg/ml  $635.00
V5 tagged protein detected with Mouse anti-V5-tag (MOUSE ANTI V5-TAG).
V5 tagged protein detected with Mouse anti-V5-Tag (MOUSE ANTI V5-TAG).

Goat Anti-V5 Tag Secondary Antibody LS-C83295
Goat Polyclonal
ELISA, ICC, IP, WB
Unconjugated
100µg  $355.00; 1mg  $1,140.00
Detection of V5-tagged fusion protein in 200, 100, and 50ng of E. coli lysate

Rabbit Anti-V5 Tag Secondary Antibody LS-C154221
Rabbit Polyclonal (IgG)
ELISA, IHC, WB
Unconjugated
100µg 1mg/ml  $335.00
Anti-V5 Epitope Tag Polyclonal Antibody - Western Blot. Anti-V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. This antibody was used at 1.0 ug/ml to detect 0.05 ug (lane 2) of full-length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS-PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code for 45 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Mouse Anti-V5 Tag Secondary Antibody (clone SV5-Pk4, FITC) LS-C188439
Mouse Monoclonal [clone SV5-Pk4] (IgG1) to Virus V5 Tag
Virus
Flo, IF
FITC Conjugated
0.1mg  $385.00

Sheep Anti-V5 Tag Secondary Antibody LS-C136566
Sheep Polyclonal (IgG)
IHC, WB
Unconjugated
500µg  $325.00

Mouse Anti-V5 Tag Secondary Antibody LS-C132843
Mouse Monoclonal (IgG1)
ELISA, IF, IP, WB
Unconjugated
100µg 1mg/ml  $495.00; 1mg  $995.00

Mouse Anti-V5 Tag Secondary Antibody (Biotin) LS-C530132
Mouse Monoclonal (IgG1)
ELISA, IF, IP, WB
Biotin Conjugated
100µl  $680.00

Mouse Anti-V5 Tag Secondary Antibody (FITC) LS-C540048
Mouse Monoclonal (IgG1)
ELISA, IF, IP, WB
FITC Conjugated
100µl  $680.00

Mouse Anti-V5 Tag Secondary Antibody (clone E10) LS-C136804
Mouse Monoclonal [clone E10] (IgG)
ELISA, IHC, WB
Unconjugated
100µg  $315.00
Western blot Image

Mouse Anti-V5 Tag Secondary Antibody (clone SV5-Pk1, Biotin) LS-C57306
Mouse Monoclonal [clone SV5-Pk1] (IgG2a) to Virus V5 Tag
Virus
ELISA, IHC-Fr, WB
Biotin Conjugated
0.1mg  $385.00

Mouse Anti-V5 Tag Secondary Antibody LS-C413457
Mouse Monoclonal
ICC, IF, IP, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunofluorescent analysis of V5-tag staining in 293T cells transfected with a V5-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of over-expressed V5-tagged protein in 293T cell lysate.

Mouse Anti-V5 Tag Secondary Antibody (clone 4C12E11) LS-C203627
Mouse Monoclonal [clone 4C12E11] (IgG2a,k)
ELISA, Flo, IP, WB
Unconjugated
40µg  $265.00; 100µg  $345.00; 200µg 0.5mg/ml  $495.00
Western blot analysis of immunoprecipitates from V5-tagged protein transfected HEK293 cell lysates using THE TM V5 Antibody, mAb, Mouse. Lane 1: V5-tagged protein transfected HEK293 cell lysate as control. Lane 2-3: Immunoprecipitates of V5-tagged protein transfected and non-transfected HEK293 cell lysate with V5 Antibody, mAb, Mouse. The signal was developed with IRDye TM 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
Superior specificity assay of transfected cell lysates using Western blot with THE™ V5 Antibody, mAb, Mouse. Lane 1: V5-tagged protein transfected CHO cell lysate. Lane 2: Non-transfected CHO cell lysate. The signal was developed with IRDye TM 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

Mouse Anti-V5 Tag Secondary Antibody (clone 1H6) LS-C179857
Mouse Monoclonal [clone 1H6] (IgG2a,k)
ICC, IP, WB
Unconjugated
100µg 1mg/ml  $355.00
Immunocytochemistry Image
Western blot Image

Rabbit Anti-V5 Tag Secondary Antibody LS-C83299
Rabbit Polyclonal
ELISA, ICC, WB
Unconjugated
100µg 1mg/ml  $355.00; 1mg  $1,140.00
Detection of V5-tagged fusion protein in 200, 100, and 50ng of E. coli lysate

Goat Anti-V5 Tag Secondary Antibody (FITC) LS-C83297
Goat Polyclonal
ICC, IF
FITC Conjugated
1mg  $2,745.00

Rabbit Anti-V5 Tag Secondary Antibody (FITC) LS-C285382
Rabbit Polyclonal (IgG)
ELISA, WB
FITC Conjugated
200µl  $740.00

Rabbit Anti-V5 Tag Secondary Antibody (HRP) LS-C51628
Rabbit Polyclonal (IgG)
ELISA, WB
HRP Conjugated
40µg  $245.00; 100µg 0.5mg/ml  $325.00
Western blot of V5-tagged fusion protein using V5-tag Antibody [HRP], pAb, Rabbit (V5-tag Antibody [HRP], pAb, Rabbit, 1 ug/ml) The signal was developed with LumiSensor HRP Substrate Kit Predicted Size: 52 kD Observed Size: 52 kD This image was taken for the unconjugated form of this product. Other forms have not been tested.

Mouse Anti-V5 Tag Secondary Antibody (clone Q31) LS-C387317
Mouse Monoclonal [clone Q31]
WB
Unconjugated
50µl  $235.00; 100µl  $265.00; 200µl  $335.00
Western blot of V5-Tag antibody

Rabbit Anti-V5 Tag Secondary Antibody LS-C758242
Rabbit Polyclonal to Rabbit V5 Tag
Rabbit
ELISA, Flo, IF, Micro
Unconjugated
100µg  $415.00

Mouse Anti-V5 Tag Secondary Antibody LS-C126453
Mouse Monoclonal (IgG1)
ELISA, IF, IP, WB
Unconjugated
100µg  $495.00; 1mg  $980.00

Mouse Anti-V5 Tag Secondary Antibody (Biotin) LS-C530133
Mouse Monoclonal (IgG1)
ELISA, IF, IP, WB
Biotin Conjugated
100µl  $680.00

Mouse Anti-V5 Tag Secondary Antibody (FITC) LS-C540049
Mouse Monoclonal (IgG1)
ELISA, IF, IP, WB
FITC Conjugated
100µl  $680.00

Mouse Anti-V5 Tag Secondary Antibody (clone SV5-Pk4) LS-C188440
Mouse Monoclonal [clone SV5-Pk4] (IgG1) to Virus V5 Tag
Virus
ELISA, IF, IP, WB
Unconjugated
100µg 1mg/ml  $305.00; 1mg  $605.00

Mouse Anti-V5 Tag Secondary Antibody (clone SV5-Pk5) LS-C188442
Mouse Monoclonal [clone SV5-Pk5] (IgG1) to Virus V5 Tag
Virus
ELISA, IF, IP, WB
Unconjugated
100µg 1mg/ml  $305.00; 1mg  $590.00

Mouse Anti-V5 Tag Secondary Antibody LS-C124767
Mouse Monoclonal (IgG2a)
ELISA, IHC, IHC-Fr, IP, RIA, WB
Unconjugated
100µg  $445.00; 1mg  $1,005.00
Showing 1-25 of 112 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).