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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNNI3 / Cardiac Troponin I Antibody (aa117‑127) LS‑C186615
Cardiac Troponin I antibody LS-C186615 is an unconjugated goat polyclonal antibody to Cardiac Troponin I (TNNI3) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C186615-100
100 µg (0.5 mg/ml)
$405
Description
Cardiac Troponin I antibody LS-C186615 is an unconjugated goat polyclonal antibody to Cardiac Troponin I (TNNI3) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Target
Human TNNI3 / Cardiac Troponin I
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 0.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
Synthetic peptide C-KVTKNITEIAD from an internal region of human TNNI3 / CTnI (NP_000354.4). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Panda, Bovine, Dog, Cat, Horse, Rabbit, Pig, Guinea pig (100%); Ferret, Opossum (91%).
Blocking Peptide
LS-E28047 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186615. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa117-127
Specificity
Human TNNI3 / CTnI
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 27+28kD bands observed in Human Heart lysates (calculated MW of 24.0kD according to NP_000354.4). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.2-0.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TNNI3 / Cardiac Troponin I
P19429 NM_000363 NP_000354.4

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Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
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Applications: Western blot, ELISA
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Applications: IHC, Western blot, Immunoprecipitation, ELISA

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Images

Western blot

TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/10/2018
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