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Catalog Number Size Price
LS-C358365-100 100 µl (1 mg/ml) $305 
TNK2 / ACK1 Antibody - Immunohistochemical analysis of ACK1 (pY284) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TNK2 / ACK1 Antibody - Immunofluorescent analysis of ACK1 (pY284) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TNK2 / ACK1 Antibody - Western blot analysis of ACK1 (pY284) expression in SHSY5Y (A); HeLa (B) whole cell lysates.
TNK2 / ACK1 Antibody - Immunohistochemical analysis of ACK1 (pY284) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TNK2 / ACK1 Antibody - Immunofluorescent analysis of ACK1 (pY284) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TNK2 / ACK1 Antibody - Western blot analysis of ACK1 (pY284) expression in SHSY5Y (A); HeLa (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human TNK2 / ACK1 Antibody (phospho‑Tyr284, IHC, IF, WB) LS‑C358365

Polyclonal Rabbit anti‑Human TNK2 / ACK1 Antibody (phospho‑Tyr284, IHC, IF, WB) LS‑C358365

Antibody:
TNK2 / ACK1 Rabbit anti-Human Polyclonal (pTyr284) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$305
LS-C358365-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
TNK2 / ACK1 Rabbit anti-Human Polyclonal (pTyr284) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
ACK1 antibody LS-C358365 is an unconjugated rabbit polyclonal antibody to ACK1 (TNK2) (pTyr284) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human TNK2 / ACK1
Synonyms
TNK2 | ACK | Activated p21cdc42Hs kinase | Ack kinase | ACK-1 | ACK1 | Activated CDC42 kinase 1 | p21cdc42Hs
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human ACK1 (pY284).
Epitope
pTyr284
Specificity
Recognizes endogenous levels of ACK1 (pY284) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNK2 / ACK1
Q07912 NM_005781 NP_005772.3

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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/1/2021