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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNK2 / ACK1

tyrosine kinase, non-receptor, 2

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.

Gene Name: tyrosine kinase, non-receptor, 2
Family/Subfamily: Protein Kinase , ACK
Synonyms: TNK2, ACK, Activated p21cdc42Hs kinase, Ack kinase, ACK-1, ACK1, Activated CDC42 kinase 1, p21cdc42Hs
Target Sequences: NM_005781 NP_005772.3 Q07912

Filtered by: None
Target
TNK2 / ACK1 (66)
Reactivity
Human (65)
Mouse (15)
Rat (11)
Bat (1)
Bovine (2)
Dog (1)
Guinea pig (1)
Hamster (1)
Horse (1)
Monkey (1)
Pig (2)
Rabbit (1)
Application
IHC (20)
IHC-P (7)
WB (48)
Flo (1)
DB (16)
ELISA (45)
ICC (1)
IF (9)
Peptide-ELISA (5)
Host
rabbit (66)
Isotype
IgG (55)
Clonality
polyclonal pc (66)
Format
AP Conjugated (5)
APC Conjugated (5)
Azide-free (4)
Biotin Conjugated (5)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (5)
Unconjugated (36)
Epitope
pTyr284 (12)
pTyr518 (9)
aa265-290 (8)
aa934-964 (8)
pTyr826 (8)
220-300 aa (2)
Asn280 (1)
aa10-39 (1)
aa161-175 (1)
aa231-280 (1)
aa250-299 (1)
aa278-289 (1)
aa383-411 (1)
aa466-515 (1)
aa814-844 (1)
pTyr857,pTyr858 (1)
Publications
No (66)
Showing 1-25 of 66 results

IHC-plus™ TNK2 / ACK1 Antibody (aa278-289) LS-B6360
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Mouse, Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-TNK2 / ACK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.25 ug/ml.
Western blot Image

TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C34825
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, WB
Unconjugated
100µl  $770.00

TNK2 / ACK1 Antibody (phospho-Tyr518) LS-C97107
Rabbit Polyclonal to Human TNK2 / ACK1
Human
DB
Unconjugated
400µl  $325.00
Dot blot of anti-Phospho-ACK1-pY518 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

TNK2 / ACK1 Antibody (aa934-964) LS-C99164
Rabbit Polyclonal to Human TNK2 / ACK1
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-ACK1 Antibody in CEM cell line lysates (35 ug/lane). ACK1(arrow) was detected using the purified antibody.

IHC-plus™ TNK2 / ACK1 Antibody LS-B12327
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
IF, IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C358365
Rabbit Polyclonal to Human TNK2 / ACK1
Mouse, Bovine, Rat, Pig, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of ACK1 (pY284) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of ACK1 (pY284) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

TNK2 / ACK1 Antibody (phospho-Tyr518) LS-C291479
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Rat, Human
ELISA, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from HepG2 cells treated with EGF, using phospho-ACK (Phospho-Tyr518) antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ACK (Phospho-Tyr518) Antibody

TNK2 / ACK1 Antibody (220-300 aa) LS-C386793
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Mouse, Human
ELISA, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of ACK antibody

TNK2 / ACK1 Antibody (aa10-39) LS-C155932
Rabbit Polyclonal to Human TNK2 / ACK1
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with ACK1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of ACK1 Antibody in K562 cell line lysates (35 ug/lane). ACK1 (arrow) was detected using the purified antibody.

TNK2 / ACK1 Antibody LS-C809625
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C198587
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.

TNK2 / ACK1 Antibody (220-300 aa) LS-C381489
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C357153
Rabbit Polyclonal to Human TNK2 / ACK1
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl  $325.00
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody.
Immunofluorescence of A549 cells, using ACK1 (Phospho-Tyr284) antibody.

TNK2 / ACK1 Antibody LS-C402095
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.7mg/ml  $205.00; 60µl 0.7mg/ml  $235.00; 120µl 0.7mg/ml  $295.00; 200µl 0.7mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human breast cancer using TNK2 Polyclonal Antibody at dilution of 1:70.
Immunohistochemistry of paraffin-embedded Human liver cancer using TNK2 Polyclonal Antibody at dilution of 1:70.

TNK2 / ACK1 Antibody (phospho-Tyr857,pTyr858) LS-C804441
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

TNK2 / ACK1 Antibody (aa934-964) LS-C326779
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

TNK2 / ACK1 Antibody (aa934-964, Biotin) LS-C274816
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IHC, WB
Biotin Conjugated
200µl  $740.00

TNK2 / ACK1 Antibody (aa934-964, FITC) LS-C274817
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IHC, WB
FITC Conjugated
200µl  $740.00

TNK2 / ACK1 Antibody (aa934-964, AP) LS-C274814
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IHC, WB
AP Conjugated
200µl  $740.00

TNK2 / ACK1 Antibody (aa934-964, PE) LS-C274820
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IHC, WB
PE Conjugated
200µl  $740.00

TNK2 / ACK1 Antibody (aa934-964, APC) LS-C274815
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IHC, WB
APC Conjugated
200µl  $740.00

TNK2 / ACK1 Antibody (aa934-964, Azide-free, HRP) LS-C274818
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

TNK2 / ACK1 Antibody LS-C659656
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
ELISA, IHC, WB
Unconjugated
100µg  $435.00

TNK2 / ACK1 Antibody LS-C341115
Rabbit Polyclonal to Human TNK2 / ACK1
Mouse, Rat, Human
IF, WB
Unconjugated
100µl 1mg/ml  $385.00

TNK2 / ACK1 Antibody LS-C771303
Rabbit Polyclonal (IgG) to Human TNK2 / ACK1
Human
IF, WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00
Showing 1-25 of 66 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).