Target
Human SUMO1 / SMT3
Synonyms
SUMO1 | GAP modifying protein 1 | GMP1 | OFC10 | Sentrin | SMT3 homolog 3 | SMT3C | SMT3H3 | SUMO-1 | Ubiquitin-like protein UBL1 | PIC1 | Ubiquitin-like 1 (sentrin) | UBL1 | DAP1 | GAP-modifying protein 1 | Ubiquitin-like protein SMT3C
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
Usage
This purified polyclonal antibody reacts with human SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. An 11.6 kD band corresponding to human SUMO is detected. Most human cell lysates can be used as a positive control without induction or stimulation.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee