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Catalog Number Size Price
LS-C59544-500 500 µg (5 mg/ml) $541 
SUMO1 / SMT3 Antibody - Anti-SUMO Antibody - Western Blot. Western blot of hSUMO fusion protein. Anti-SUMO antibody, generated by immunization with recombinant human SUMO, was tested by western blot against a SUMO-GFP fusion protein after cleavage by proteases. Dilution of the antibody between 1:1000 and 1:5000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead). In this blot the antibody was used at a 1:2000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # LS-C60943) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www. lifesensors. com, personal communication.

Polyclonal Rabbit anti‑Human SUMO1 / SMT3 Antibody (WB) LS‑C59544

Polyclonal Rabbit anti‑Human SUMO1 / SMT3 Antibody (WB) LS‑C59544

Antibody:
SUMO1 / SMT3 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$541
LS-C59544-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SUMO1 / SMT3 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMT3 antibody LS-C59544 is an unconjugated rabbit polyclonal antibody to human SMT3 (SUMO1). Validated for ELISA and WB.
Target
Human SUMO1 / SMT3
Synonyms
SUMO1 | GAP modifying protein 1 | GMP1 | OFC10 | Sentrin | SMT3 homolog 3 | SMT3C | SMT3H3 | SUMO-1 | Ubiquitin-like protein UBL1 | PIC1 | Ubiquitin-like 1 (sentrin) | UBL1 | DAP1 | GAP-modifying protein 1 | Ubiquitin-like protein SMT3C
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:25000)
Usage
This purified polyclonal antibody reacts with human SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. An 11.6 kD band corresponding to human SUMO is detected. Most human cell lysates can be used as a positive control without induction or stimulation.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SUMO1 / SMT3
P63165 NM_003352 NP_003343.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/10/2022