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Catalog Number Size Price
LS-C745301-25 25 µl (1 mg/ml) $275 
SMAD3 Antibody - Affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
SMAD3 Antibody - Western blot using the affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein.
SMAD3 Antibody - Affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
SMAD3 Antibody - Western blot using the affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein.
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Polyclonal Rabbit anti‑Human SMAD3 Antibody (phospho‑Ser423/425, IHC, WB) LS‑C745301

Polyclonal Rabbit anti‑Human SMAD3 Antibody (phospho‑Ser423/425, IHC, WB) LS‑C745301

Antibody:
SMAD3 Rabbit anti-Human Polyclonal (pSer423/425) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus laevis, Xenopus tropicalis
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745301-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
SMAD3 Rabbit anti-Human Polyclonal (pSer423/425) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus laevis, Xenopus tropicalis
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-C745301 is an unconjugated rabbit polyclonal antibody to SMAD3 (pSer423/425) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB.
Target
Human SMAD3
Synonyms
SMAD3 | HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | HSMAD3 | Mad protein homolog | SMAD 3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus laevis, Xenopus tropicalis (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein.
Epitope
pSer423/425
Specificity
Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • IHC (1:500)
  • Western blot (1:2000 - 1:20000)
  • Flow Cytometry
  • ELISA (1:75000 - 1:300000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021